The Effects Of Protease-Activated Receptors 1 And 4 In Human Platelet Activation And Inflammation:

Foot & Ankle Orthopaedics

JianyingZhang¹ 

关键词: Tendon Injury;    Platelet-Rich Plasma (PRP);    Protease-Activated Receptors (PAR);    PAR 1;    PAR 4;    HMGB-1;    IL-1RA;    VEGF;    Endostatin;   

DOI  :  10.1177/2473011416S00297

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Category:Basic Sciences/BiologicsIntroduction/Purpose:Tendon injuries occur frequently and cost billions of health care dollars annually. Recently, there has been an increase in the use of platelet-rich plasma (PRP) to treat tendon injuries. However, the efficacy of PRP treatment is controversial due to inconsistent results from human clinical trials. It is thought that variations in PRP preparation contribute to these inconsistencies. Specifically, platelets in PRP contain pro-angiogenic (e.g. VEGF) or anti-angiogenic (e.g. endostatin) factors, which may differentially affect the healing of tendon injuries. It is known that these factors are selectively released after platelet activation by specific receptors. Therefore, in this study we investigated the effect of protease-activated receptors 1 and 4 (PAR1 and PAR4) in platelet activation and inflammation.Methods:Platelet preparation – Human blood was obtained from 12 healthy donors and 9 ml of blood was mixed with 1 ml of 3.8% sodium citrate and centrif...

【 授权许可】

CC BY