Enzyme catalytic promiscuity: asymmetric aldol addition reaction catalyzed by a novel thermophilic esterase in organic solvent

Green Chemistry Letters and Reviews

Wang Haoran¹ 

关键词: catalytic promiscuity;    asymmetric aldol addition;    2-butanone;    4-nitrobenzaldehyde;    enantioselectivity;   

DOI  :  10.1080/17518253.2014.902507

学科分类：化学（综合）

来源: Taylor & Francis

【 摘 要 】

The asymmetric aldol addition of 2-butanone and 4-nitrobenzaldehyde catalyzed by a novel thermophilic esterase (APE1547) from the archaeon Aeropyrum pernix K1 was successfully conducted in organic solvents. APE1547 exhibited a good enzyme activity and enantioselectivity in the reaction. The effects of organic solvent, temperature, water content, and substrate concentration were investigated. The reaction provided optically active secondary alcohol with satisfying enantioselectivity (71.2 %ee) and enzyme activity (38.1 µmol/g/h) under the optimum conditions. A high yield (68.7%) could be obtained when the reaction time was approximately 120 h.

【 授权许可】

Unknown