Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study:

Biomedical Engineering and Computational Biology

DavidTes¹ 

关键词: Fluorescence imaging;    Cy5;    CRANAD-2;    amyloid-β;    Alzheimer disease;    early diagnosis;    instrumentation;   

DOI  :  10.1177/1179597218781081

学科分类：工程和技术（综合）

来源: Sage Journals

【 摘 要 】

Alzheimer disease is the most common form of dementia, affecting more than 5 million people in the United States. During the progression of Alzheimer disease, a particular protein begins to accumulate in the brain and also in extensions of the brain, ie, the retina. This protein, amyloid-β (Aβ), exhibits fluorescent properties. The purpose of this research article is to explore the implications of designing a fluorescent imaging system able to detect Aβ proteins in the retina. We designed and implemented a fluorescent imaging system with a range of applications that can be reconfigured on a fluorophore to fluorophore basis and tested its feasibility and capabilities using Cy5 and CRANAD-2 imaging probes. The results indicate a promising potential for the imaging system to be used to study the Aβ biomarker. A performance evaluation involving ex vivo and in vivo experiments is planned for future study.

【 授权许可】

CC BY