Molecular cytogenetic identification of a novel hexaploid wheat-Thinopyrum intermedium partial amphiploid with high protein content

Turkish Journal of Biology

MARIYANA GEORGIEVA¹ 

关键词: FISH;    GISH;    wheat-Thinopyrum intermedium introgression line;   

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat × Thinopyrum intermedium (wheatgrass) cross. This line is a high-protein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of the genomic type of wheatgrass chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B, and D genomes of wheat were not observed. FISH using the repetitive DNA probes pSc119.2, Afa-family, and pTa71 allowed for identification of all the wheat chromosomes present.

【 授权许可】

Unknown