A Method to Detect Differential Gene Expression in Cross-Species Hybridization Experiments at Gene and Probe Level:

Biomedical Informatics Insights

YingChen¹ 

关键词: gene expression;    cross-species;    probes;    genes;    hybridization;   

DOI  :  10.4137/BII.S3846

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

MotivationWhole genome microarrays are increasingly becoming the method of choice to study responses in model organisms to disease, stressors or other stimuli. However, whole genome sequences are available for only some model organisms, and there are still many species whose genome sequences are not yet available. Cross-species studies, where arrays developed for one species are used to study gene expression in a closely related species, have been used to address this gap, with some promising results. Current analytical methods have included filtration of some probes or genes that showed low hybridization activities. But consensus filtration schemes are still not available.ResultsA novel masking procedure is proposed based on currently available target species sequences to filter out probes and study a cross-species data set using this masking procedure and gene-set analysis. Gene-set analysis evaluates the association of some priori defined gene groups with a phenotype of interest. Two methods, Gene Set ...

【 授权许可】

CC BY