The motogenic effect of EGF and TGF-α on the migration of tumor cells from the oral region: A role of epithelial-to-mesenchymal transition in cancer and a route for translation into the clinic

Translational Research in Oral Oncology

MohammadIslam¹ 

关键词: EGF;    TGF-α;    EMT;    head;    neck cancer;   

DOI  :  10.1177/2057178X17698481

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Aim:Epithelial-to-mesenchymal transition (EMT) is recognized as a hallmark of cancer. The change in phenotype of epithelial cells enables them to migrate and the cancer therefore, to become more aggressive. The presence of the epidermal growth factor receptor (EGFR) has been implicated in the spread and aggressive nature of oral tumors. The aims of this present study were to investigate the role of two different growth factors, both ligands of the receptor, and their influence on EMT and cellular motility.Methods:A number of assays were used to investigate the response of the cells to the two growth factors (EGF and transforming growth factor (TGF-α)). These techniques included the 2-D scratch assay, a 2-D-cell scatter assay, and immunocytochemistry. Normal keratinocytes (HaCaT), oral adenoid squamous cell carcinoma (TYS), and human salivary gland tumor cells were used in this study.Results:The results of the scratch and scatter assays indicated that EGF and TGF-α exerted differential effects upon the cel...

【 授权许可】

CC BY