Characterization of GABAergic Marker Expression in the Chronic Unpredictable Stress Model of Depression:

Chronic Stress

MouniraBanasr¹ 

关键词: depression;    chronic stress;    gamma-aminobutyric acid;    GAD67;    calcium-binding proteins;    neuropeptides;   

DOI  :  10.1177/2470547017720459

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

BackgroundEvidence continues to build suggesting that the GABAergic neurotransmitter system is altered in brains of patients with major depressive disorder. However, there is little information available related to the extent of these changes or the potential mechanisms associated with these alterations. As stress is a well-established precipitant to depressive episodes, we sought to explore the impact of chronic stress on GABAergic interneurons.MethodsUsing western blot analyses and quantitative real-time polymerase chain reaction, we assessed the effects of five-weeks of chronic unpredictable stress exposure on the expression of GABA-synthesizing enzymes (GAD65 and GAD67), calcium-binding proteins (calbindin, parvalbumin, and calretinin), and neuropeptides co-expressed in GABAergic neurons (somatostatin, neuropeptide Y, vasoactive intestinal peptide, and cholecystokinin) in the prefrontal cortex and hippocampus of rats. We also investigated the effects of corticosterone and dexamethasone exposure on the...

【 授权许可】

CC BY