Effect of essential oils of clove and cumin against the growth of Staphylococus Aureus isolated from Denture Stomatitis

Padjadjaran Journal of Dentistry

Minasari M.¹ 

[1] Universitas Sumatera Utara

关键词: essential oils;    clove;    cumin;    Staphylococcus Aureus;    Denture Stomatitis;   

DOI  :  10.24198/pjd.vol28no1.13509

学科分类：口腔科学

来源: Universitas Padjadjaran

【 摘 要 】

Background:Essential oils of clove and cumin had an inhibition effect againstStaphylococcus aureus . Clove’s essential oils has a compound named eugenol, which can directly damage the membrane cell of bacteria. Thymoquinone, the active ingredient in the black cumin’s essential oils inhibits the protein synthesis and cause malfunction of the bacterial cell. The purpose of this research was to determine the differences of inhibitory effect from essential oils of cloves and cumin to the growth ofStaphylococcus aureus .Method:This research was an experimental laboratory with Post-test Only Control Group Design. Sample that being used for this experiment wasStaphylococcus aureusthat had been isolated from a denture stomatitis patient. This inhibition test was determined using a Disc Diffusion Test’s method with the essential oils of clove and cumin, while distilled water and 96% ethanol as a negative and positive control, respectively. Essential oils were obtained from the distillation method with water and steam and the test was done 7 times repetition with every ingredients. Inhibition zone was measured with a vernier calipers. The data were analyzed by ANOVA One-way test followed by a multiple comparison test.Result:   The average zone of inhibition againstStaphylococcus aureusfrom aquades 0 mm, 96% ethanol 13.894 mm, the essential oils of clove 14.784 mm and black cumin 11.944 mm. The multiple comparison test analysis showed a significant differences (p <0.05) between the average zone of inhibition of the materials tested.Conclusion:Clove essential oil has a greater inhibition againstStaphylococcus aureusthan the essential oils of cumin.

【 授权许可】

CC BY