Production of 2,3-butanediol by Enterobacter cloacae from corncob-derived xylose

Turkish Journal of Biology

CUIYING ZHANG¹ 

关键词: Fermentation;    cellulose;    2;    3-butanediol;    bioreactors;    Enterobacter cloacae;    microbial growth;   

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

During the process of industrial production of biofuels and biochemicals from lignocellulosic biomass, large amounts of waste byproducts rich in xylose are generated, resulting in excessive wastage of natural resources and environmental pollution. In this work, xylose solution from corncob hydrolysate was utilized to produce 2,3-butanediol (2,3-BD), using the strain Enterobacter cloacae CICC 10011 to improve the utilization rate of hemicellulose and reduce environmental pollution. 2,3-BD fermentation conditions were subsequently developed. Xylose solution and (NH4)2HPO4, selected with the Plackett-Burman experiment, were determined as significant independent variables to conduct a response surface experiment. With the optimized medium, 50.02 g/L 2,3-BD production was obtained, which corresponded to 85.16% of the theoretical value. Furthermore, 81.4 g/L 2,3-BD production, 92.95 g/L the total production (2,3-BD + acetoin), and 0.72 g/(L h) productivity were obtained by fed-batch fermentation. Therefore, efficient production of 2,3-BD from corncob-derived xylose is important in the future development and expansion of biorefining technologies.

【 授权许可】

Unknown