Allergen Profile of Protophormia terraenovae, Other Species of Calliphoridae, and Lumbricus terrestris in Anglers Allergic to Maggots in Cáceres, Spain

Journal of Investigational Allergology and Clinical Immunology

FJ Hernández Arbeiza¹  MC Diéguez Pastor²  B de la Hoz Caballer²  M Alvarado Arenas¹  S Jiménez Timón¹  E Rodríguez Martín¹  AB Sánchez González⁵  EM Frontera Carrión³  F Pineda de la Losa⁴  SL Porcel Carreño¹ 

[1] Sección de Alergología, Complejo Hospitalario de Cáceres, Cáceres, Spain;Servicio de Alergología, Hospital Ramón y Cajal, Madrid, Spain;Departamento de Parasitología, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain;R+D+I Department, Diater laboratorios, SA, Madrid, Spain;Centro de Salud de Alcántara, Área de Salud de Cáceres, Alcántara, Spain

关键词: Fish bait;    Anglers;    Allergy;    Allergens;    Maggot;    Protophormia terraenovae;   

Others  :  1183809

【 摘 要 】

Background: Our group previously found that up to 7% of amateur anglers in Caceres, Spain may be allergic to the larvae of Protophormia terraenovae (order Diptera, family Calliphoridae) used as live bait for fishing.

Objective: To identify the pattern of major allergens in P terraenovaeand other species of Calliphoridae.

Materials and Methods: Extracts of P terraenovae, Calliphora vomitoria,Lucilia sericataand Lumbricus terrestris were characterized using sodium dodecyl sulfate polyacrylamide gel electrophoresis and IgE-immunoblotting techniques in individual sera from 24 patients with a positive skin test result and/or specific IgE determination (enzyme-linked immunosorbent assay [ELISA]) to P terraenovae. ELISA and IgE-immunoblotting inhibition studies were also performed to identify potential cross-reactive allergens between these species.

Results: IgE-immunoblotting with P terraenovae showed a band of 15.3 kDa recognized by 15 patients, in addition to 2 further allergens of 22.8 kDa and 69 kDa. For C vomitoria, 5 bands of 73, 46, 40, 28, and 14 kDa were observed. ForL sericata, 2 major allergens of 73 kDa and 14 kDa were observed. In the case of L terrestris, IgE from 13 patients recognized 1 allergen of around 15.5 kDa. IgE-immunoblotting and ELISA inhibition revealed the presence of cross-reactivity, mainly between L terrestris andP terraenovae.

Conclusions:P terraenovae appears to have species-specific allergens and allergens shared with C vomitoriaand L sericata. Striking immunological cross-reactivity was observed between P terraenovae and L terrestris. An allergen of 15-16 kDa could be involved in this phenomenon.