Cytoadherence characteristics to endothelial receptors ICAM-1 and CD36 of Plasmodium falciparum populations from severe and uncomplicated malaria cases

Parasite

and R. Durand²  J. Le Bras²  S. Jafari-Guemouri³  S. Cojean¹ 

[1] Centre National de Référence pour la Chimiosensibilité du Paludisme (CNRCP), Hôpital Bichat Claude Bernard, 46, rue Henri Huchard,75018 Paris,France;Laboratoire de Parasitologie Mycologie, Hôpital Avicenne et EA 3406, Université Paris 13,Bobigny,France;UR 010, Mother and child Health in the Tropics, Institut de Recherche pour le Développement,Paris,France

关键词: msp-2;    CD36;    ICAM-1;    cytoadherence;    uncomplicated malaria;    severe malaria;    Plasmodium falciparum;   

Others  :  808548
DOI  :  doi:10.1051/parasite/2008152163

 received in 2007-09-10, accepted in 2008-03-20,  发布年份 2008

【 摘 要 】

The adhesion of infected red blood cells (IRBCs) to the cell lining of microvasculature is thought to play a central role in the pathogenesis of severe malaria. Individual IRBC can bind to more than one host receptor and parasites with multiple binding phenotypes may cause severe disease more frequently. However, as most clinical isolates are multiclonal, previous studies were hampered by the difficulty to distinguish whether a multiadherent phenotype was due to one or more parasite population(s). We have developed a tool, based on cytoadhesion assay and GeneScan genotyping technology, which enabled us to assess on fresh isolates the capacity of adherence of individual P. falciparum genotypes to human receptors expressed on CHO transfected cells. The cytoadhesion to ICAM-1 and CD36 of IRBCs from uncomplicated and severe malaria attacks was evaluated using this methodology. In this preliminary series conducted in non immune travelers, IRBCs from severe malaria appeared to adhere more frequently and/or strongly to ICAM-1 and CD36 in comparison with uncomplicated cases. In addition, a majority genotype able to strongly adhere to CD36 was found more frequently in isolates from severe malaria cases. Further investigations are needed to confirm the clinical relevance of these data.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2008