Establishment of Eimeria tenella (local isolate) in chicken embryos

Parasite

L. Jiang Q. Zhao S. Zhu H. Han H. DongB. Huang¹ 

[1] Key Laboratory for Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai 200241,China

关键词: vaccine;    in vitro cultivation;    embryo;    chicken;    Eimeria tenella;   

Others  :  808802
DOI  :  doi:10.1051/parasite/2012193285

 received in 2011-02-08, accepted in 2012-06-04,  发布年份 2012

【 摘 要 】

Development of an in vitro Eimeria (E.) tenella model could be valuable as a tool for vaccine, coccidiostats or molecular biology research. 1.0 × 104 sporozoites per 0.1 mL were inoculated into the allantoic cavity of ten-day-old chicken embryos. The complete lifecycle of E. tenella was accomplished in eight-nine days at 37 °C and 70% humidity. The addition of 100 U insulin to the embryos could remarkably improve the output of oocysts. The development of the parasite within the embryos was systematically observed, allowing guidelines to be set regarding the appropriate times at which different developmental stages of the parasite may be sampled.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2012

【 图 表 】

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