A stage-specific open reading frame from three-day old adult worms of Trichinella spiralis encodes zinc-finger motifs

Parasite

and P. Boireau⁴  G.G. Ortega-Pierres⁵  M.Y. Liu²  Y.P. Yang³  C.V. Li⁴  J. Yang³  B.Q. Fu²  P. Garcia-Reyna¹  X.P. Zhu³ 

[1] Facultad de Estudios Superiores Cuautitlán, UNAM,México;Changchun University of Agriculture and Animal Sciences,Changchun,130062,PR China;Department of Parasitology, School of Basic Medicine, Capital University of Medical Sciences,Beijing,100054,PR China;JRU Molecular Biology and Immunology of Parasites and Fungi, INRA AFSSA ENVA UPVM,Maisons Alfort,France;Department of Genetics and Molecular Biology, Cinvestav,México

关键词: cloning;    DNA sequencing;    cDNA library;    three-day old adult;    Trichinella spiralis;   

Others  :  808785
DOI  :  doi:10.1051/parasite/2005122151

 received in 2004-07-21, accepted in 2005-01-11,  发布年份 2005

【 摘 要 】

The aim of the study was to isolate genes coding for stage-specific antigens of T. spiralis. Such antigens may then be associated with local and systemic immune responses against adult T. spiralis. Recombinant clones were obtained with an adult stage specific probe from a cDNA library of three-day old adult T. spiralis. Several cDNA clones encoding the same peptide were identified and their stage specificity was confirmed by northern blot analysis. Three independent clones were fully sequenced, and the resulting sequence found to code for a 487 amino acid peptide with a deduced molecular weight of ≈ 55 kDa. Sequence analysis showed that the 55 kDa peptide contained putative DNA binding motifs, suggesting that this protein may be involved in transcriptional regulation during the early development of the parasite.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2005