Study of the sustained speed of kill of the combination of fipronil/amitraz/(S)-methoprene and the combination of imidacloprid/permethrin against Dermacentor reticulatus, the European dog tick

Parasite

and M.G. Pollmeier²  C. Ollagnier¹  F. Beugnet²  J.J. Fourie³ 

[1] Merial S.A.S., PIPA, CRSV, 1, allée des Cyprès,01150 Saint-Vulbas,France;Merial S.A.S., 29, avenue Tony Garnier,69007 Lyon,France;ClinVet, P.O. Box 11186, Universitas,Bloemfontein,9321,Republic of South Africa

关键词: CERTIFECT®;    ADVANTIX®;    permethrin;    imidacloprid;    (S)-methoprene;    amitraz;    fipronil;    Dermacentor reticulates;   

Others  :  808844
DOI  :  doi:10.1051/parasite/2011184319

 received in 2011-07-01, accepted in 2011-07-13,  发布年份 2011

【 摘 要 】

The sustained speed of kill against Dermacentor reticulatus of two topical combinations, one containing fipronil/amitraz/(S)-methoprene and the other, imidacloprid/permethrin, was evaluated in dogs. Two treated groups and one untreated control group of eight adult Beagle dogs each were randomly formed based on pre-infestation rates and bodyweight. Each treatment was administered topically once on Day 0, according to the recommended label dose and instructions for use. All dogs were infested with 50 adult unfed D. reticulatus starting on Day 1, then weekly, for a total of five weeks. While most studies determine tick efficacy at 48 hours (h), in this study, all remaining ticks were counted and categorized 24 h following each infestation. The numbers of ticks (living or dead) that remained attached on treated dogs were compared to those on the control animals. The percent reduction of attached ticks (disruption of attachment) at 24 h on dogs treated with fipronil/amitraz/(S)-methoprene remained above 92% for four weeks. The reduction of attached ticks at 24 h on dogs treated with imidacloprid/permethrin did not reach 80% during the entire study. The number of ticks attached at 24 h was significantly (p < 0.05) lower in the fipronil/ amitraz/(S)-methoprene group than in the imidacloprid/permethrin group in assessments on Days 2, 15, 22, 29 and 36. When assessing efficacy based upon live ticks on treated versus control dogs, fipronil/amitraz/(S)-methoprene 24 h efficacy was above 95% for four weeks, decreasing to 77.8% at Day 36. The 24 h efficacy of imidacloprid/permethrin ranged from 56.2% to 86.7% through Day 29, never achieving 90% throughout the study. The 24-hour efficacy of fipronil/amitraz/(S)-methoprene was significantly (p < 0.05) higher than imidacloprid/permethrin at all time points, including Day 36.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2011

【 图 表 】

Fig 1.

Fig 2.

【 参考文献 】

• [1]Berrada Z.L. & Telford III S.R. Burden of tick-borne infections on American companion animals. Topics in Companion Animal Medicine, 2009, 24, 175–181. [PubMed]
• [2]Beugnet F. & Marie J.L. Emerging arthropod borne diseases of companion animals in Europe. Veterinary Parasitology, 2009, 163, 298–305. [PubMed]
• [3]Brianti E., Pennisi M.G., Brucato G., Risitano A.L., Gaglio G., Lombardo G., Malara D., Fogliazza A. & Giannetto S. Efficacy of the fipronil 10% + (S)-methoprene 9% combination against Rhipicephalus sanguineus in naturally infested dogs: speed of kill, persistant efficacy on immature and adult stages and effect of water. Veterinary Parasitology, 2010, 170, 96–103. [PubMed]
• [4]Doyle V., Beugnet F. & Carithers D. Comparative efficacy of the combination fipronil-(S)-methoprene and the combination permethrin-imidacloprid against Dermacentor reticulatus, the European dog tick, applied topically to dogs. Veterinary Therapeutics, 2005, 6 (4), 303–310.
• [5]Dryden M.W., Payne P.A. Smith V. & Hostetler J. Evaluation of an imidacloprid (8.8% w/w)-permethrin (44% w/w) topical spot-on and a fipronil (9.8% w/w)-(S)-methoprene (8.8%w/w) topical spot on to repel, prevent attachment, and kill adult Rhipicephalus sanguineus and Dermacentor variabilis ticks on dogs. Veterinary Therapeutics, 2006, 7, 187–198.
• [6]Dryden M.W., Payne P.A., Mcbride A., Mailen S., Smith V. & Carithers D. Efficcay of fipronil (9.8% w/w) + (S)- methoprene (8.8% w/w) and imidacloprid (8.8% w/w) + permethrin (44% w/w) against Dermacentor variabilis (American dog tick) on dogs. Veterinary Therapeutics, 2008, 9 (1), 15–25.
• [7]Marchiondo A.A., Holdsworth P.A., Green P., Blagburn B.L. & Jacobs D.E. World Association for the Advancement of Veterinary Parasitology (WAVVP) guidelines for evaluating the efficacy of parasiticides for the treatment, prevention and control of flea and tick infestation on dogs and cats. Veterinary Parasitology, 2007, 145, 332–344. [PubMed]
• [8]Nicholson W.L., Allen K.E., Mcquiston J.H., Breitschwerdt E.B. & Little S.E. The increasing recognition of rickettsial pathogens in dogs and people. Trends in Parasitology, 2010, 26, 205–212. [PubMed]
• [9]Pfister K. Fipronil, amitraz and (S)-methoprene – a novel ectoparasiticide combination for dogs. Veterinary Special Edition, 2011, 179 (4), 291–353.
• [10]Prullage J., Hair J., Everett W., Yoon S.Y., Cramer L., Franke S., Cornelison M. & Hunter J. The prevention of attachment and the detachment effects of a novel combination of fipronil, amitraz and (S)-methoprene for Rhipicephalus sanguineus and Dermacentor variabilis on dogs. Veterinary Special Edition, 2011, 179 (4), 302–310.
• [11]Prullage J.B., Cawthorne W.G., Le Hir De Fallois L.P. & Timmons P.R. Synergy between fipronil and amitraz in a Rhipicephalus sanguineus tick residual contact test, Experimental & Applied Acarology, 2011, 54, 173–176. [PubMed]
• [12]Tielemans E., Manavella C., Pollmeier M., Chester T., Murphy M. & Gale B. Comparative acaricidal efficacy of the topically applied combinations fipronil/(S)-methoprene, permethrin/ imidacloprid and metaflumizone/amitraz against Dermacentor reticulatus, the Eurpean dog tick (Ornate dog tick, Fabricius, 1794) in dogs. Parasite, 2010, 17, 343–348. [PubMed]