Consequence of temperature changes on cercarial shedding from Galba truncatula infected with Fasciola hepatica or Paramphistomum daubneyi

Parasite

and Gilles Dreyfuss²  Abdeslam Mekroud¹  Philippe Vignoles²  Amal Titi¹  Daniel Rondelaud² 

[1] PADESCA Laboratory, Veterinary Science Institute,25100 El-Khroub,Constantine,Algeria;INSERM U 1094, Faculties of Medicine and Pharmacy,87025 Limoges,France

关键词: Temperature change;    Paramphistomum daubneyi;    Galba truncatula;    Fasciola hepatica;    Cercarial shedding;    Cercaria;   

Others  :  808629
DOI  :  doi:10.1051/parasite/2013009

 received in 2012-10-12, accepted in 2013-02-22,  发布年份 2013

【 摘 要 】

Experimental infections of Galba truncatula (two populations) with Fasciola hepatica or Paramphistomum daubneyi were carried out to study the effect of water temperature changes (3 h at a mean of 12 °C every week) on cercarial shedding during the patent period. The results were compared with those of control snails infected according to the same protocol and always maintained at 20 °C. Compared to controls, a significant increase in the number of cercariae-shedding snails, a significantly longer patent period and significantly greater cercarial production were noted in temperature-challenged snails, regardless of the type of digenean infection. In contrast, the number of incompletely formed metacercariae was significantly higher in temperature-challenged snails than in controls. Incompletely formed metacercariae of F. hepatica consisted of cysts whose colour remained whitish after shedding (25.4% for temperature-challenged snails) or whose dome was flattened after encystment (74.6%). Those of P. daubneyi were totally dark brown or blackish after formation. These incomplete metacercariae might originate from young differentiating cercariae within the snail body (F. hepatica) or from cercariae which died just after encystment (P. daubneyi). The use of regular temperature changes for snails infected with F. hepatica or P. daubneyi must be monitored carefully during collection of metacercariae to select completely formed cysts for infecting definitive hosts.

【 授权许可】

   
© D. Rondelaud et al., published by EDP Sciences, 2013

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Abrous M, Rondelaud D, Dreyfuss G. 1999a. Influence of low temperatures on the cercarial shedding of Paramphistomum daubneyi from the snail Lymnaea truncatula. Parasite, 6, 85–88.
• [2]Abrous M, Rondelaud D, Dreyfuss G. 1999b. Paramphistomum daubneyi and Fasciola hepatica: influence of low temperatures on the shedding of cercariae from dually infected Lymnaea truncatula. Parasitology Research, 85, 765–769.
• [3]Andrews SJ. 1999. The life cycle of Fasciola hepatica, in Fasciolosis, Dalton JP, Editor. CABI Publishing: Oxon, 1–29.
• [4]Audousset JC, Rondelaud D, Dreyfuss G, Vareille-Morel C. 1989. Les émissions cercariennes de Fasciola hepatica L. chez le mollusque Lymnaea truncatula Müller. A propos de quelques observations chronobiologiques. Bulletin de la Société Française de Parasitologie, 7, 217–224.
• [5]Caron Y, Lasri S, Losson B. 2007. Fasciola hepatica: a study of the vectorial capacity of four different species of lymnaeid snails commonly found in Belgium. Veterinary Parasitology, 149, 95–103. [PubMed]
• [6]Dixon KE. 1966. A morphological and histochemical study of the cystogenic cells of the cercaria of Fasciola hepatica L. Parasitology, 56, 287–297. [PubMed]
• [7]Graczyk TK, Fried B. 1999. Development of Fasciola hepatica in the intermediate host, in Fasciolosis. Dalton JP, Editor. CABI Publishing: Oxon, p. 31–46.
• [8]Hodasi JKM. 1972. The output of cercariae of Fasciola hepatica by Lymnaea truncatula and the distribution of metacercariae on grass. Parasitology, 65, 431–436.
• [9]Kendall SB, McCullough FS. 1951. The emergence of the cercariae of Fasciola hepatica from the snail Lymnaea truncatula. Journal of Helminthology, 25, 77–92.
• [10]Mercer EH, Dixon KE. 1967. The fine structure of the cystogenic cells of the cercaria of Fasciola hepatica L. Zeitschrift für Zellforschung und Mikroskopische Anatomie, 77, 331–344.
• [11]Mud Snail Study Group. Omphiscola glabra captive breeding programme.Release strategy, 2006, Website: http://www.ephemeroptera.pwp.blueyonder.co.uk/mssg/1869 (Consulted on March 22, 2012).
• [12]Ollerenshaw CB. 1971. Some observations on the epidemiology of fascioliasis in relation to the timing of molluscicide applications in the control of the disease. Veterinary Record, 88, 152–164. [PubMed]
• [13]Rondelaud D, Vignoles P, Dreyfuss G. 2009. La Limnée tronquée, un mollusque d’intérêt médical et vétérinaire. PULIM: Limoges.
• [14]Rondelaud D, Fousi M, Vignoles P, Moncef M, Dreyfuss G. 2007. Optimization of metacercarial production for three digenean species by the use of Petri dishes for raising lettuce-fed Galba truncatula. Parasitology Research, 100, 861–865. [PubMed]
• [15]Smyth JD, Halton DW. 1983. The physiology of trematodes. Cambridge University Press: Cambridge.
• [16]Taylor EL. 1965. Fascioliasis and the liver-fluke, 64. FAO Agricultural Studies: Roma.
• [17]Vignoles P, Dreyfuss G, Rondelaud D. 2002. Larval development of Fasciola hepatica in experimental infections: variations with populations of Lymnaea truncatula. Journal of Helminthology, 76, 179–183. [PubMed]
• [18]Vignoles P, Alarion N, Bellet V, Dreyfuss G, Rondelaud D. 2006. A 6–8 day periodicity in cercarial shedding occurred in some Galba truncatula experimentally infected with Fasciola hepatica. Parasitology Research, 98, 385–388. [PubMed]