Caractérisation moléculaire et morphologique de deux espèces affines de Paraphlebotomus: Phlebotomus chabaudi Croset, Abonnenc & Rioux, 1970 et P. riouxi Depaquit, Killick-Kendrick & Léger, 1998 (Diptera : Psychodidae)

Parasite

et J. Depaquit⁴  S. Berchi²  H. Babba¹  H. Ferté⁴  D. Augot⁴  D. Jouet⁴  R. Boudabous¹  A. Bounamous³ 

[1] Laboratoire de Parasitologie-Mycologie, code 99UR/08-05, Faculté de Pharmacie, Département de Biologie Clinique B, 1, Rue Avicenne,5000 Monastir Tunisie;Laboratoire de Biosystématique et Ecologie des Arthropodes, Faculté des Sciences de la Nature et de la Vie, Université Mentouri,25000 Constantine,Algérie;Laboratoire de pharmacologie et phytochimie, Faculté des Sciences de Jijel,Algérie;JE 2533-USC AFSSA “VECPAR”, Faculté de Pharmacie, Université de Reims Champagne-Ardenne,Reims,France

关键词: taxonomy;    molecular biology;    Tunisia;    Algeria;    phlebotomine sandflies;   

Others  :  808440
DOI  :  doi:10.1051/parasite/2008154565

【 摘 要 】

In 1998, the description of Phlebotomus riouxi emphasised the difficulty to differentiate the female from the closely related P. chabaudi, a suspected vector of Leishmania killicki in several foci in Tunisia. In order to be able to distinguish the females of these two species, we started a molecular study based on 37 Algerian and Tunisian specimens. The alignment of the sequences of the cytochrome b of the mitochondrial DNA and their analysis using Neighbor-Joining, maximum likelihood and maximum parsimony shows the individualisation of two species including an intraspecific variability. Following a morphological approach, it is not possible to distinguish the females on the basis of their spermathecae. A new character is proposed: the presence of antero-lateral teeth of the pharyngeal armature for P. chabaudi, never observed in P. riouxi. However, a molecular typing is necessary at the present time for a sure identification of the females.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2008