Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco

Parasite

and G. Schönian³  I. Abbasi¹  A. Nasereddin¹  M.Z. Alam³  H. Fellah²  F. Sebti²  M. Rhajaoui² 

[1] Department of Biology, University Al-Quds,Jerusalem,Palestine;Département de parasitologie, Institut National d’Hygiène,Rabat,Morocco;Institut fuer Mikrobiologie und Hygiene, Charité Universitaetsmedizin,Berlin,Germany

关键词: Morocco;    ITS1-PCR;    Leishmania tropica;    leishmaniasis;   

Others  :  808836
DOI  :  doi:10.1051/parasite/2012191081

 received in 2011-04-17, accepted in 2011-09-10,  发布年份 2012

【 摘 要 】

Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in this region. Species identification was achieved by performing the ITS1-PCR-RFLP approach. By using this method it was possible to identify parasites in Giemsa stained slides containing less than five parasites per oil-immersion field even they were conserved for up to four months.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2012

【 图 表 】

Fig. 1.

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