Lymnaea palustris and Lymnaea fuscus are potential but uncommon intermediate hosts of Fasciola hepatica in Sweden

Particle and Fibre Toxicology

Johan Höglund³  Daniel Rondelaud⁴  Luboš Beran¹  Martin Kašný²  Adam Novobilský³ 

[1] Agency for Nature Conservation and Landscape Protection of the Czech Republic, Kokořínsko Protected Landscape Area Administration, Česká 149, Mělník 276 01, Czech Republic;Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, Brno 611 37, Czech Republic;Department of Biomedical Sciences and Veterinary Public Health, Section for Parasitology, Swedish University of Agricultural Sciences (SLU), Uppsala 750 07, Sweden;INSERM U 1094, Faculty of Medicine and Faculty of Pharmacy, 2 rue du Docteur Raymond Marcland, Limoges 87025, France

关键词: Shell size;    Metacercariae;    Lymnaeidae;    Lymnaea palustris;    Lymnaea fuscus;    ITS-2;    Intermediate host;    Galba truncatula;    Fascioloides;    Fasciola hepatica;   

Others  :  824725
DOI  :  10.1186/1756-3305-6-251

 received in 2013-06-04, accepted in 2013-08-26,  发布年份 2013

【 摘 要 】

Background

Lymnaea palustris and L. fuscus are members of the European stagnicolines (Gastropoda: Lymnaeidae). The role of stagnicolines in transmission of Fasciola hepatica has been often proposed. To assess the possible relationship between these two stagnicolines and F. hepatica in Sweden, field monitoring in parallel with experimental infections of L. palustris and L. fuscus were conducted.

Methods

Stagnicoline snails were collected and identified on pastures grazed by either sheep or cattle on four farms suffering from fasciolosis in Sweden during 2011–2012. Field-collected L. palustris and L. fuscus were examined for F. hepatica DNA by PCR. In the laboratory, different age groups of L. palustris, L. fuscus and G. truncatula were each exposed to two F. hepatica miracidia and main infection characteristics were obtained.

Results

One field-collected L. palustris (out of n = 668) contained F. hepatica as determined by PCR. On the other hand, stagnicolines artificially exposed to F. hepatica miracidia resulted in successful infection with fully differentiated cercariae, but only in juvenile snails (size, 1–2 mm at exposure) and with a prevalence of 51% and 13% in L. palustris and L. fuscus, respectively. In contrast, 90% of juvenile (size, 1–2 mm) and 92% of preadult G. truncatula (size, ≥ 2-4 mm), respectively, were successfully infected. Delayed, reduced and/or no spontaneous cercarial shedding was observed in the two stagnicolines when compared to G. truncatula. However, at snail dissection most cercariae from L. fuscus and L. palustris were able to encyst similarly to those from G. truncatula.

Conclusion

Both L. fuscus and L. palustris can sustain larval development of F. hepatica but with an apparent level of age resistance. The finding of a single F. hepatica positive specimen of L. palustris, together with infection characteristics from the experimental infection, suggest that L. palustris is a more suitable snail vector of F. hepatica than L. fuscus. The reduced growth observed in both stagnicolines was contrary to the ‘parasitic gigantism’ theory. Overall, it seems that the epidemiological role of L. palustris in transmission of F. hepatica in Sweden is likely to be much lower than for G. truncatula.

【 授权许可】

   
2013 Novobilský et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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