期刊论文详细信息
Virology Journal
Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification
Xiang-tao Liu1  Yong-sheng Liu1  Jie Zhang1  Hao-tai Chen1 
[1] State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Key laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, P.R.China
关键词: Specificity;    Sensitivity;    Reverse transcription loop-mediated isothermal amplification;    Detection;    Foot-and-mouth disease virus;   
Others  :  1155651
DOI  :  10.1186/1743-422X-8-510
 received in 2011-07-19, accepted in 2011-11-09,  发布年份 2011
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【 摘 要 】

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus. Furthermore, the assay correctly detected 84 FMDV positive samples but not 65 FMDV negative specimens. The result indicated the potential usefulness of the technique as a simple and rapid procedure for the detection of FMDV infection.

【 授权许可】

   
2011 Chen et al; licensee BioMed Central Ltd.

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