Use of ex vitro composite plants to study the interaction of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

Plant Methods

Michael P Timko¹  Ava M Hoffman¹  Karolina E Mellor¹ 

[1] Department of Biology, University of Virginia, Gilmer Hall 044, Charlottesville, VA 22903, USA

关键词: Witchweed;    Striga gesnerioides;    Resistance;    Hairy roots;    Composite plants;    Agrobacterium rhizogenes;   

Others  :  822220
DOI  :  10.1186/1746-4811-8-22

 received in 2012-05-16, accepted in 2012-06-21,  发布年份 2012

【 摘 要 】

Background

Cowpea (Vigna unguiculata L.) is an important grain and forage legume grown throughout sub-Saharan Africa primarily by subsistence farmers on poor, drought prone soils. Genetic improvement of the crop is being actively pursued and numerous functional genomics studies are underway aimed at characterizing gene controlling key agronomic characteristics for disease and pest resistances. Unfortunately, similar to other legumes, efficient plant transformation technology is a rate-limiting step in analysis of gene function in cowpea.

Results

Here we describe an optimized protocol for the rapid generation of transformed hairy roots on ex vitro composite plants of cowpea using Agrobacterium rhizogenes. We further demonstrate the applicability of cowpea composite plants to study gene expression involved in the resistance response of the plant roots to attack by the root parasitic weed, Striga gesnerioides. The utility of the new system and critical parameters of the method are described and discussed herein.

Conclusions

Cowpea composite plants offer a rapid alternative to methods requiring stable transformation and whole plant regeneration for studying gene expression in resistance or susceptibility responses to parasitic weeds. Their use can likely be readily adapted to look at the effects of both ectopic gene overexpression as well as gene knockdown of root associated defense responses and to the study of a broader range of root associated physiological and aphysiological processes including root growth and differentiation as well as interactions with other root pests, parasites, and symbionts.

【 授权许可】

   
2012 Mellor et al.; licensee BioMed Central Ltd.

【 图 表 】

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