Different cDNA microarray patterns of gene expression reflecting changes during metastatic progression in adenoid cystic carcinoma

World Journal of Surgical Oncology

Weiliu Qiu²  Zhiyuan Zhang²  Fan Yu²  Ronggen He²  Wantao Chen²  Dan Huang¹ 

[1] Department of Pathology, Virginia Commonwealth University, Richmond, VA, USA;Laboratory of Cancer Biology, Shanghai Second Medical University, Shanghai, P. R. China

关键词: adenoid cystic;    carcinoma;    tumor;    neoplasm;    salivary gland;    expression;    gene;    cell line;    metastasis;    cDNA microarray;   

Others  :  1207493
DOI  :  10.1186/1477-7819-1-28

 received in 2003-08-30, accepted in 2003-12-19,  发布年份 2003

【 摘 要 】

Background

The metastatic ability of tumor cells is determined by level of expression of specific genes that may be identified with the aid of cDNA microarray containing thousands of genes and can be used to establish the expression profile of disease related genes in complex biological system.

Materials and Methods

Salivary adenoid cystic carcinoma cell line and its high metastases adenoid cystic carcinoma clone were used as model systems to reveal the gene expression alteration related to metastasis mechanism by cDNA microarray analysis. The correlation of metastatic phenotypic changes and expression levels of 4 selected genes (encoding CD98, L6, RPL29, and TSH) were further validated by using RT-PCR analysis of human tumor specimens from primary adenoid cystic carcinoma and corresponding metastasis lymph nodes.

Results

Of the 7,675 clones of known genes and expressed sequence tags (ESTs) that were analyzed, 30 showed significantly different (minimum 3 fold) expression levels in two cell lines. Out of 30 genes found differentially expressed, 18 were up regulated (with ratio more than 3) and 12 down regulated (with ratio less than 1/3).

Conclusion

Some of these genes are known to be involved in human tumor antigen, immune surveillance, adhesion, cell signaling pathway and growth control. It is suggested that the microarray in combination with a relevant analysis facilitates rapid and simultaneous identification of multiple genes of interests and in this study it provided a profound clue to screen candidate targets for early diagnosis and intervention.

【 授权许可】

   
2003 Huang et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

【 图 表 】

Figure 1.

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