Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice

Stem Cell Research & Therapy

Ali Mojallal⁴  Odile Damour¹  Dominique Sigaudo-Roussel¹  Héléna Rutschi³  Orianne Ardisson¹  Ondine Rouyer¹  Audrey Josset-Lamaugarny¹  Charlotte Lequeux¹  Fabien Boucher⁴  Jonathan Rodriguez² 

[1] IBCP-UMR 5305 CNRS, 7 passage du Vercors, Lyon, 69 367, Cedex 07, France;Cell and Tissue Bank, Cutaneous Substitute Laboratory, Edouard Herriot Hospital, 5, place d’Arsonval, Pavillon I, Lyon, 69437, France;Laboratoire Central d’Anatomie Pathologique, Hôpital Édouard Herriot, Lyon, France;Service de chirurgie plastique, esthétique et reconstructrice, Hospices Civils de Lyon, University of Lyon, Lyon, France

关键词: Vehicle;    Skin blood perfusion;    Cutaneous wound healing;    Adipose-derived stem cells;   

Others  :  1235619
DOI  :  10.1186/s13287-015-0238-3

 received in 2014-07-09, accepted in 2015-11-16,  发布年份 2015

【 摘 要 】

Background

The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential effect of ASCs on the healing of 12 mm diameter-excisional wounds (around 110 mm ² ) in nude mice.

Methods

Thirty nude mice underwent surgery to create one 12-mm excisional wound per mouse (spontaneous healing, n = 6; Cytocare® 532, n = 12; ASCs, n = 12). The Galiano wound model was chosen to avoid shrinkage and thus slow the spontaneous healing (SH) of mouse skin, making it closer to the physiology of human skin healing. Transparent dressings were used to enable daily healing time measurements to be taken. Immunohistochemistry, histological and blood perfusion analysis were carried out on the healed skin.

Results

The in vivo results showed the effectiveness of using ASCs on reducing the time needed for complete healing to 21.2 days for SH, 17.4 days for vehicle alone (Cytocare® 532) and 14.6 days with the addition of ASCs (p < 0.001). Moreover, cutaneous perfusion of the healed wound was significantly improved in ASC-treated mice compared to SH group, as shown by laser Doppler flowmetry and the quantitation of blood vessels using immunohistochemistry of αsmooth muscle actin.

Conclusions

The tolerance and efficacy of cryopreserved ASCs to accelerate the complete closure of the wound by increasing the maturation of the skin and its blood perfusion, shows their therapeutic benefit in the wound healing context.

【 授权许可】

   
2015 Rodriguez et al.

【 图 表 】

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