Airborne spread and infection of a novel swine-origin influenza A (H1N1) virus

Virology Journal

Tongjie Chai⁵  Zengmin Miao¹  Peiqiang Hou³  Huili Yang³  Jing Gao⁵  Hao Wang⁵  Mingchao Cui⁵  Baozhi Wei⁵  Zhihao Liu⁵  Mingliang Zhang⁵  Qinglei Li⁵  Xinxian Li⁵  Hongliang Dong⁵  Yufa Zhou⁶  Xiaoxia Li¹  Ruihua Ma⁴  Xin Li⁵  Hongna Zhang² 

[1] Taishan Medical University, Tai’an, China;Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China;Centre for Disease Control, Tai’an, the People’s Republic of China, Tai’an, China;Affiliated Hospital of the Shandong Agricultural University, Tai’an, China;College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, Tai’an 271018, China;The Animal Husbandry Bureau of Tai’an City, Tai’an, China

关键词: Guinea pig;    Pig;    Airborne transmission;    Epidemic;    S-O 2009 IV;   

Others  :  1149472
DOI  :  10.1186/1743-422X-10-204

 received in 2013-01-05, accepted in 2013-05-07,  发布年份 2013

【 摘 要 】

Background

The novel swine-origin influenza A (H1N1) virus (S-O 2009 IV) can cause respiratory infectious diseases in humans and pigs, but there are few studies investigating the airborne spread of the virus. In January 2011, a swine-origin H1N1 epidemic emerged in eastern China that rapidly spread to neighboring farms, likely by aerosols carried by the wind.

Methods

In this study, quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect viruses in air samples from pig farms. Based on two aerosol infection models (Pig and guinea pig), we evaluated aerosol transmission and infection of the novel S-O 2009 IV isolate.

Results

Three novel S-O 2009 IV were isolated from the diseased pig. The positive rate and viral loads of air samples were 26.1% and 3.14-5.72 log₁₀copies/m³ air, respectively. In both pig and guinea pig infection models, the isolate (A/swine/Shandong/07/2011) was capable of forming aerosols and infected experimental animals at a range of 2.0-4.2 m by aerosols, but aerosol route was less efficient than direct contact.

Conclusions

The results indicated that S-O 2009 IV is able to be aerosolized by infected animals and to be transmitted to susceptible animals by airborne routes.

【 授权许可】

   
2013 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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