Site directed biotinylation of filamentous phage structural proteins

Virology Journal

Jonathan M Gershoni¹  Larisa Smelyanski¹ 

[1] Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978 Israel

关键词: combinatorial libraries;    phage display;    biotinylation;    filamentous bacteriophage;   

Others  :  1155731
DOI  :  10.1186/1743-422X-8-495

 received in 2011-07-03, accepted in 2011-11-01,  发布年份 2011

【 摘 要 】

Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E. coli) into the N termini of four of the five structural proteins of the filamentous bacteriophage fd (Proteins 3, 7, 8 and 9). The in vivo and in vitro biotinylation of the various phages were compared. The production of multifunctional phages and their application as affinity reagents are demonstrated.

【 授权许可】

   
2011 Smelyanski and Gershoni; licensee BioMed Central Ltd.

【 图 表 】

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