Prevalence of canine leishmaniasis in Beichuan County, Sichuan, China and phylogenetic evidence for an undescribed Leishmania sp. in China based on 7SL RNA

Particle and Fibre Toxicology

Jian-Ping Chen¹  Da-Li Chen¹  Bin-Bin Yang²  Lin Liao¹  Yu Tian¹  Ya-Jing Wang¹  Jian-Guo Zhang¹  Wang Guan³  Ke Sun¹ 

[1] Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041, China;Institute of Laboratory Medicine, Ningxia Medical University, Yinchuan, 750004, China;Department of Parasitology, North Sichuan Medical College, Nanchong, Sichuan, 637000, China

关键词: 7SL RNA;    Enzyme-linked immunosorbent assay (ELISA);    rK39 dip-stick;    Phylogeny;    Canine leishmaniasis;    Prevalence;   

Others  :  1232496
DOI  :  10.1186/1756-3305-5-75

 received in 2012-02-21, accepted in 2012-04-19,  发布年份 2012

【 摘 要 】

Background

Leishmaniasis is a vector-borne disease, which is still endemic in the west and northwest area of China. Canines are the major reservoirs of Leishmania, the etiological agent of human visceral leishmaniasis. Phlebotomus chinensis is the main transmission vector of zoonotic visceral leishmaniasis (ZVL).

Methods

In this study, rK39 dip-stick, ELISA and PCR methods were used to investigate the prevalence of canine leishmaniasis (CanL) in Beichuan County, Sichuan Province, China.

Results

Among the 86 dogs which were included in the study, 13 dogs were positive using the dip-stick test (15.12%), while 8 dogs were positive using ELISA (9.30%) and 19 dogs were positive for PCR (22.03%). In total, 32 dogs were positive for one or more tests (37.21%). Interestingly, phylogenetic analysis based on the partial 7SL RNA fragment provided evidence that an undescribed Leishmania species, which is clearly a causative agent of CanL and human visceral leishmaniasis, does exist in China. This result is consistent with our previous study.

Conclusions

Our work confirmed that canine leishmaniasis is still prevalent in Beichuan County. Further control is urgently needed, as canine leishmaniasis is of great public health importance. The phylogenetic analysis based on 7SL RNA segment provides evidence for the existence of an undescribed Leishmania sp. in China.

【 授权许可】

   
2012 Sun et al.; licensee BioMed Central Ltd.

【 图 表 】

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Figure 1.

Figure 2.

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