Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China

Particle and Fibre Toxicology

Quan Liu¹  Xing-Quan Zhu³  Si-Yang Huang²  Fu-Kai Zhang²  Ze-Dong Wang¹  Nan Li¹  Ye Liu¹  Wei Cong⁴  Si-Yuan Qin⁴ 

[1] Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, Jilin Province, PR China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu Province, PR China;Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou 225009, Jiangsu Province, PR China;College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Jilin Province, PR China

关键词: China;    Genetic characterization;    Prevalence;    Bats;    Toxoplasma gondii;   

Others  :  1148808
DOI  :  10.1186/s13071-014-0558-7

 received in 2014-08-28, accepted in 2014-11-23,  发布年份 2014

【 摘 要 】

Background

Toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. Limited information about T. gondii infection in bats is available in China. The objective of the present study was to determine prevalence and genetic characterization of T. gondii infection in bats in Jilin, Liaoning, Jiangxi and Guangdong provinces, China.

Methods

During May 2005 to August 2013, bats were sampled from Jilin, Liaoning, Jiangxi, and Guangdong provinces, China, and liver tissues were collected for the detection of T. gondii by a nested PCR targeting the B1 gene. The positive samples were genotyped at 11 genetic markers (SAG1, 5′-and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results

A total of 626 bats representing 10 species were examined for T. gondii infection, 38 (6.1%) were tested positive with by PCR, 8 positive DNA samples were completely genotyped, of which 3 samples (2 from Cynopterus sphinx, and 1 from Murina leucogaster) represented ToxoDB#10, and 5 samples (2 from Murina leucogaster, 2 from Myotis chinensis, and 1 from Rhinolophus ferrumequinum) belonged to ToxoDB#9 (http://toxodb.org/toxo/ webcite).

Conclusions

The present study revealed an overall T. gondii prevalence of 6.1% in bats from Jilin, Liaoning, Jiangxi and Guangdong provinces in China, and reported two T. gondii genotypes (ToxoDB#9 and #10) having a wide geographical distribution in China. These results provide new genetic information about T. gondii infection in bats, and have implications for better understanding of the genetic diversity of T. gondii in China and elsewhere.

【 授权许可】

   
2014 Qin et al.; licensee BioMed Central Ltd.

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