Changes in receptivity epithelial cell markers of endometrium after ovarian stimulation treatments: its role during implantation window

Reproductive Health

Marco Cerbón⁴  Yanira Franco-Murillo⁴  Jesús Chimal-Monroy¹  Juan C. Hinojosa Cruz³  Victor S. Vital-Reyes³  Armando Gamboa-Domínguez²  Francisco J. Valdez-Morales⁴ 

[1] Instituto Nacional de Perinatología, México, D.F., Mexico;Departamento de Patología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D.F., Mexico;Departamento de Biología de la Reproducción, Hospital de Ginecología y Obstetricia # 3, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, México, D.F., Mexico;Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México, México, D.F., Mexico

关键词: Receptivity markers;    Recombinant follicle stimulating hormone;    Clomiphene citrate;    Ovulation induction;    Human endometrium;    Infertility;   

Others  :  1210978
DOI  :  10.1186/s12978-015-0034-7

 received in 2014-10-27, accepted in 2015-04-30,  发布年份 2015

【 摘 要 】

Background

To compare the expression of receptivity markers in epithelial and stromal cells in the endometrium of ovulatory women and infertile with hypothalamic pituitary dysfunction (HPD), untreated or treated with clomiphene citrate (CC), or with recombinant follicle stimulating hormone (rFSH).

Methods

Twelve control ovulatory and 32 anovulatory women, 22 of whom received ovulation induction with CC (n = 12) or rFSH (n = 10). Endometrial biopsies were obtained during the mid-secretory phase. Hormonal secretion was measured by chemiluminescence immunoassay, endometrial dating and cellular expression and distribution of receptivity proteins were evaluated by quantitative immunohistochemistry.

Results

CC or rFSH treatments, modified the expression of epithelial receptivity markers, such as Glycodelin A, beta-catenin, CD166/ALCAM and IGF-1R, but not in stromal markers. Also, a change in their cell distribution was observed.

Conclusions

Treatment of infertile women with HPD modified the expression and distribution of receptivity markers in the mid-secretory phase of the endometrium in epithelial but not stromal cells, which can help to explain changes in the receptivity of the endometrium during treatments and suggest an important role of these cells in the receptivity window.

【 授权许可】

   
2015 Valdez-Morales et al.; licensee BioMed Central.

【 图 表 】

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