Prospective cohort study of the circadian rhythm pattern in allogeneic sibling donors undergoing standard granulocyte colony-stimulating factor mobilization

Stem Cell Research & Therapy

Paul S Frenette³  Lorraine K Miller¹  James H Godbold²  Erin L Moshier²  Janice L Gabrilove¹  Luis M Isola¹  Patricia A Shi¹ 

[1] Tisch Cancer Institute, Mount Sinai School of Medicine, 1190 5th Avenue, New York, NY, 10029, USA;Department of Preventive Medicine, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY, 10029, USA;Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine, Albert Einstein College of Medicine, 1301 Morris Park Avenue, Bronx, NY, 10461, USA

关键词: Hematopoietic stem cell mobilization;    Hematopoietic progenitor cells;    Granulocyte-colony stimulating factor;    Circadian rhythm;    CD38;    Antigens;    CD34;    Antigens;   

Others  :  1179376
DOI  :  10.1186/scrt180

 received in 2012-11-20, accepted in 2013-03-12,  发布年份 2013

【 摘 要 】

Introduction

Prior in vivo murine studies suggest circadian oscillations for hematopoietic stem cell release, which are maintained following administration of granulocyte colony-stimulating factor (G-CSF) or plerixafor. Furthermore, retrospective data analysis of healthy donors who underwent G-CSF-induced mobilization demonstrated significantly increased CD34⁺ cell yields when collected in the afternoon compared with the morning.

Methods

A prospective study was conducted to directly examine the number of peripheral blood CD34⁺ and CD34⁺CD38^– progenitor/stem cells at baseline and then every 6 hours for 24 hours on days 4 to 5 of G-CSF (10 μg/kg/day in the morning) mobilization in 11 allogeneic donors. Data were analyzed using mixed-model analysis of repeated measures.

Results

Whereas we observed a significant increase in CD34⁺ cell counts toward the evening, counts were then sustained on the morning of day 5. The correlation between CD34⁺CD38^– cell counts and the less defined CD34⁺ populations was weak.

Conclusions

Our results suggest that the pharmacodynamic activity and timing of G-CSF may alter endogenous progenitor rhythms. Donor age, medical history, and medications may also impact circadian rhythm. Further studies should examine the circadian rhythm at the peak of G-CSF mobilization and should consider potential confounders such as the time of G-CSF administration and the age of the subjects.

【 授权许可】

   
2013 Shi et al.; licensee BioMed Central Ltd.

【 图 表 】

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