A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis

Particle and Fibre Toxicology

Patrícia Sampaio Tavares Veras²  Alexandre Barbosa Reis⁴  Selma Maria Bezerra Jerônimo³  Geraldo Gileno de Sá Oliveira²  Glória Regina Monteiro³  Wendel Coura-Vital⁴  Isaac Queiroz de Oliveira⁵  Lairton Souza Borja⁵  Luciano Vasconcellos Pacheco⁵  Edimilson Domingos da Silva¹  Deborah Bittencourt Mothé Fraga² 

[1] Laboratório de Tecnologia Diagnóstica, Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil;Instituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT, Bahia, Brazil;Departamento de Bioquimica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil;Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil;Laboratório de Patologia e Biointervenção, Centro de Pesquisa Gonçalo Moniz, FIOCRUZ, Salvador, Bahia, Brazil

关键词: Multicentric study;    Rapid test;    Recombinant antigen;    Serological diagnosis;    Canine visceral leishmaniasis;   

Others  :  807681
DOI  :  10.1186/1756-3305-7-136

 received in 2013-10-29, accepted in 2014-03-16,  发布年份 2014

【 摘 要 】

Background

Visceral leishmaniasis (VL) is a serious public health challenge in Brazil and dogs are considered to be the main urban reservoir of the causative agent. The culling of animals to control VL in some countries makes the accurate diagnosis of canine VL (CVL) essential. Recombinant antigens rLci1A and rLci2B were selected from a cDNA library of Leishmania infantum amastigotes due to their strong potential as candidates in diagnostic testing for CVL. The present multicentric study aimed to evaluate the sensitivity of a prototype test using these antigens (DPP rLci1A/rLci2B) against 154 sera obtained from symptomatic dogs within three endemic areas of VL in Brazil. The specificity was evaluated using 40 serum samples from negative dogs and dogs infected with other pathogens. Sensitivity and specificity rates of DPP rLci1A/rLci2B prototype were compared to rates from other diagnostic tests currently in use by the Brazilian Ministry of Health, including DPP®LVC, EIE®LVC.

Findings

DPP rLci1A/rLci2B prototype offered similar performance to that offered by DPP®LVC rapid test, as follows: sensitivity of 87% (CI 81–91) and 88% (CI 82–93) and specificity of 100% (CI 91–100) and 97% (CI 87–100), respectively for DPP rLci1A/rLci2B and DPP®LVC. When results of these two tests were considered concomitantly, sensitivity increased to 93.5% (CI 89–96).

Conclusions

The recombinant antigens rLci1A and rLci2B represent promising candidates for use in a multi-antigen rapid test for CVL. The inclusion of novel antigens to the DPP rLci1A/rLci2B prototype model could offer additionally enhanced sensitivity to detect animals infected by L. infantum.

【 授权许可】

   
2014 Fraga et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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