Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice

Particle and Fibre Toxicology

Marcelo Sousa Silva²  Diego M A Guérin⁴  Gerardo A Marti³  Jon Agirre¹  Jailson F B Querido² 

[1] C Fundación Biofísica Bizkaia, Barrio Sarriena S/N, Leioa, Bizkaia, 48940, Spain;Centre for Malaria and Tropical Diseases - Instituto de Higiene e Medicina Tropical - Universidade Nova de Lisboa, Lisboa, Portugal;Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET - UNLP) 2-584, La Plata, 1900, Argentina;Departamento de Bioquímica y Biologia Molecular, Universidad del País Vasco (UPV/EHU), 48940, Leioa, Spain

关键词: Mice immune response;    Trypanosoma cruzi;    triatomines;    Chagas disease;    Triatoma virus;    Dicistroviridae;   

Others  :  1228037
DOI  :  10.1186/1756-3305-6-66

 received in 2013-01-11, accepted in 2013-03-13,  发布年份 2013

【 摘 要 】

Background

Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family. Due to its peculiar genome organization and characteristics of the per os viral transmission route, dicistroviruses make good candidates for use as biopesticides. Triatoma virus (TrV) is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of the human trypanosomiasis disease called Chagas disease. TrV was postulated as a potential control agent against Chagas’ vectors. Although there is no evidence that TrV nor other dicistroviruses replicate in species outside the Insecta class, the innocuousness of these viruses in humans and animals needs to be ascertained.

Methods

In this study, RT-PCR and ELISA were used to detect the infectivity of this virus in Mus musculus BALB/c mice.

Results

In this study we have observed that there is no significant difference in the ratio IgG2a/IgG1 in sera from animals inoculated with TrV when compared with non-inoculated animals or mice inoculated only with non-infective TrV protein capsids.

Conclusions

We conclude that, under our experimental conditions, TrV is unable to replicate in mice. This study constitutes the first test to evaluate the infectivity of a dicistrovirus in a vertebrate animal model.

【 授权许可】

   
2013 Querido et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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