Generation and characterization of a recombinant Newcastle disease virus expressing the red fluorescent protein for use in co-infection studies

Virology Journal

Patti J Miller²  De-shan Li⁴  Diego G Diel²  Qingzhong Yu²  Haixia Hu³  Jinnan Li¹ 

[1] Heilongjiang Fisheries Research Institute, Harbin, 150070, China;USDA-ARS, Southeast Poultry Research Laboratory, 934 College Station Road, Athens, GA 30605, USA;College of Animal Science and Technology, Southwest University, 2 Tiansheng Road, Chongqing, BeiBei District, 400715, China;College of Life Sciences, Northeast Agriculture University, Harbin, 150030, China

关键词: Interference;    Super infection;    Co-infection;    NDV;   

Others  :  1153598
DOI  :  10.1186/1743-422X-9-227

 received in 2012-05-10, accepted in 2012-09-26,  发布年份 2012

【 摘 要 】

Background

Many viruses have evolved multiple strategies to prevent super infection of host cells by more than one virion. This phenomenon, known as super infection exclusion, may play an important role on virus evolution because it can affect the frequency of reassortment and/or recombination. Newcastle disease virus (NDV), a negative sense single-stranded RNA virus, is characterized by its continuous evolutionary dynamics and by a low frequency of recombination events. However, the mechanisms that contribute to the low recombination rates on NDV are still not completely understood.

Methods

In this study we assessed the ability of two NDV strains (LaSota and B1) to super infect host cells in vitro. We generated a recombinant NDV strain LaSota expressing the red fluorescent protein (RFP) and used it in co-infection assays with a related NDV strain B1 expressing the green fluorescent protein (GFP). DF-1 cells were inoculated with both viruses at the same time or at different intervals between primary infection and super infection.

Results

When both viruses were inoculated at the same time point, a 27% co-infection rate was observed, whereas when they were inoculated at different time points the super infection rates decreased to levels as low as 1.4%.

Conclusions

These results indicate that although different NDV strains can co-infect host cells in vitro, the super infection rates are low, specially as the time between the primary infection and super infection increases. These results confirm the occurrence of super infection exclusion between different strains of NDV.

【 授权许可】

   
2012 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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