Long-term interdisciplinary therapy reduces endotoxin level and insulin resistance in obese adolescents

Nutrition Journal

Ana R Dâmaso⁵  Lila M Oyama⁵  Claudia M Oller do Nascimento²  Marília Seelaender¹  Marco T de Mello³  Sergio Tufik³  Lian Tock²  Claudio T de Souza⁴  Aline de Piano²  Priscila L Sanches²  June Carnier²  Ronaldo V Santos⁵  Gustavo D Pimentel²  Jose C Rosa²  Fábio S Lira⁴ 

[1] Da Universidade Federal de São Paulo–UNIFESP, São Paulo. Grupo de Metabolismo e Câncer, ICB-Universidade de São Paulo–USP, São Paulo, Brazil;Departamento de Fisiologia, Rua Botucatu nº 862 Vila Clementino, São Paulo, Brazil;Departamento de Psicobiologia, Rua Marselhesa n Vila Clementino, São Paulo, Brazil;Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, SC, Brazil;Departamento de Biociências, Rua Marselhesa n Vila Clementino, São Paulo, Brazil

关键词: Interdisciplinary therapy;    Insulin resistance;    Endotoxin;    Cytokines;    Obesity;   

Others  :  823979
DOI  :  10.1186/1475-2891-11-74

 received in 2012-01-27, accepted in 2012-09-11,  发布年份 2012

【 摘 要 】

Aim

The purpose of the present study was to assess the dietary fat intake, glucose, insulin, Homeostasis model assessment for insulin resistance HOMA-IR, and endotoxin levels and correlate them with adipokine serum concentrations in obese adolescents who had been admitted to long-term interdisciplinary weight-loss therapy.

Design

The present study was a longitudinal clinical intervention of interdisciplinary therapy. Adolescents (n = 18, aged 15–19 y) with a body mass index > 95th percentile were admitted and evaluated at baseline and again after 1 year of interdisciplinary therapy. We collected blood samples, and IL-6, adiponectin, and endotoxin concentrations were measured by ELISA. Food intake was measured using 3-day diet records. In addition, we assessed glucose and insulin levels as well as the homeostasis model assessment for insulin resistance (HOMA-IR).

Results

The most important finding from the present investigation was that the long-term interdisciplinary lifestyle therapy decreased dietary fat intake and endotoxin levels and improved HOMA-IR. We observed positive correlations between dietary fat intake and endotoxin levels, insulin levels, and the HOMA-IR. In addition, endotoxin levels showed positive correlations with IL-6 levels, insulin levels and the HOMA-IR. Interestingly, we observed a negative correlation between serum adiponectin and both dietary fat intake and endotoxin levels.

Conclusions

The present results indicate an association between dietary fat intake and endotoxin level, which was highly correlated with a decreased pro-inflammatory state and an improvement in HOMA-IR. In addition, this benefits effect may be associated with an increased adiponectin level, which suggests that the interdisciplinary therapy was effective in improving inflammatory pathways.

【 授权许可】

   
2012 Lira et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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