Nutrition Journal
The effects of changing dairy intake on trans and saturated fatty acid levels- results from a randomized controlled study
Ralph AH Stewart2  Jocelyne R Benatar1 
[1]Cardiovascular Research Unit, Greenlane Cardiovascular Service, Auckland City Hospital, Auckland 1030, New Zealand
[2]Green Lane Cardiovascular Service, Auckland City Hospital, Auckland, New Zealand
关键词: Randomized controlled study;    Palmitelaidic acid;    Vaccenic acid;    Dairy food;    Saturated fatty acids;    Trans fatty acids;   
Others  :  801582
DOI  :  10.1186/1475-2891-13-32
 received in 2013-11-26, accepted in 2014-03-28, published in 13
【 摘 要 】


Dairy food is an important natural source of saturated and trans fatty acids in the human diet. This study evaluates the effect of dietary advice to change dairy food intake on plasma fatty acid levels known to be present in milk in healthy volunteers.


Twenty one samples of whole fat dairy milk were analyzed for fatty acids levels. Changes in levels of plasma phospholipid levels were evaluated in 180 healthy volunteers randomized to increase, not change or reduce dairy intake for one month. Fatty acids were measured by gas chromatography–mass spectrometry and levels are normalized to d-4 alanine.


The long chain fatty acids palmitic (13.4%), stearic (16.7%) and myristic (18.9%) acid were most common saturated fats in milk. Four trans fatty acids constituted 3.7% of the total milk fat content. Increased dairy food intake by 3.0 (± 1.2) serves/ day for 1 month was associated with small increases in plasma levels of myristic (+0.05, 95% confidence level-0.08 to 0.13, p = 0.07), pentadecanoic (+0.014, 95% confidence level -0.016 to 0.048, p = 0.02) and margaric acid (+0.02, -0.03 to 0.05, p = 0.03). There was no significant change in plasma levels of 4 saturated, 4 trans and 10 unsaturated fatty acids. Decreasing dairy food intake by 2.5 (± 1.2) serves per day was not associated with change in levels of any plasma fatty acid levels.


Dietary advice to change dairy food has a minor effect on plasma fatty acid levels.

Trial registration


【 授权许可】

2014 Benatar and Stewart; licensee BioMed Central Ltd.

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【 参考文献 】
  • [1]World Health Statistics http://apps.who.int/gho/data/node.main.1?lang=en webcite
  • [2]IDF Diabetes Atlas http://www.idf.org/diabetesatlas webcite
  • [3]Reduced or modified dietary fat for preventing cardiovascular disease Cochrane Database Syst Rev 2011, CD002137. doi:002110.001002/14651858.CD14002137.pub14651852
  • [4]Mozaffarian D, Katan MB, Ascherio A, Stampfer MJ, Willett WC: Trans fatty acids and cardiovascular disease. N Engl J Med 2006, 354:1601-1613.
  • [5]de Goede J, Verschuren WM, Boer JM, Verberne LD, Kromhout D, Geleijnse JM: N-6 and N-3 fatty acid cholesteryl esters in relation to fatal CHD in a Dutch adult population: a nested case–control study and meta-analysis. PLoS One 2013, 8:e59408.
  • [6]Lemaitre RN, King IB, Mozaffarian D, Sotoodehnia N, Rea TD, Kuller LH, Tracy RP, Siscovick DS: Plasma phospholipid trans fatty acids, fatal ischemic heart disease, and sudden cardiac death in older adults: the cardiovascular health study. Circulation 2006, 114:209-215.
  • [7]Khaw KT, Friesen MD, Riboli E, Luben R, Wareham N: Plasma phospholipid fatty acid concentration and incident coronary heart disease in men and women: the EPIC-Norfolk prospective study. PLoS Med 2012, 9:e1001255.
  • [8]Mansson HL: Fatty acids in bovine milk fat. Food Nutr Res 2008, 52:10-34.
  • [9]Diet, nutrition and the prevention of chronic diseases-Report of the joint WHO/FAO expert consultation http://www.who.int/dietphysicalactivity/publications/trs916/summary/en/ webcite
  • [10]Parodi PW: Distribution of isomeric octadecenoic fatty acids in milk fat. J Dairy Sci 1976, 59:1870-1873.
  • [11]Elwood PC: Time to value milk. Int J Epidemiol 2005, 34:1160-1162.
  • [12]Mozaffarian D, Aro A, Willett WC: Health effects of trans-fatty acids: experimental and observational evidence. Eur J Clin Nutr 2009, 63(Suppl 2):S5-S21.
  • [13]Field CJ, Blewett HH, Proctor S, Vine D: Human health benefits of vaccenic acid. Appl Physiol Nutr Metab 2009, 34:979-991.
  • [14]Bendsen NT, Christensen R, Bartels EM, Astrup A: Consumption of industrial and ruminant trans fatty acids and risk of coronary heart disease: a systematic review and meta-analysis of cohort studies. Eur J Clin Nutr 2011, 65:773-783.
  • [15]Jakobsen MU, Overvad K, Dyerberg J, Heitmann BL: Intake of ruminant trans fatty acids and risk of coronary heart disease. Int J Epidemiol 2008, 37:173-182.
  • [16]O’Donnell-Megaro AM, Barbano DM, Bauman DE: Survey of the fatty acid composition of retail milk in the United States including regional and seasonal variations. J Dairy Sci 2011, 94:59-65.
  • [17]Zock PL, Katan MB: Hydrogenation alternatives: effects of trans fatty acids and stearic acid versus linoleic acid on serum lipids and lipoproteins in humans. J Lipid Res 1992, 33:399-410.
  • [18]Perk J, De Backer G, Gohlke H, Graham I, Reiner Z, Verschuren M, Albus C, Benlian P, Boysen G, Cifkova R, Deaton C, Ebrahim S, Fisher M, Germano G, Hobbs R, Hoes A, Karadeniz S, Mezzani A, Mezzani A, Prescott E, Ryden L, Scherer M, Syvanne M, op Reimer WJ S, Vrints C, Wood D, Zamorano JL, Zannad F: European Guidelines on cardiovascular disease prevention in clinical practice (version 2012). The Fifth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of nine societies and by invited experts). Developed with the special contribution of the European Association for Cardiovascular Prevention & Rehabilitation (EACPR). Eur Heart J 2012, 33(17):1635-1701.
  • [19]Ludwig DS, Willett WC: Three daily servings of reduced-fat milk: an evidence-based recommendation? JAMA Pediatr 2013, 1-2.
  • [20]US ChooseMyPlate http://www.choosemyplate.gov/ webcite
  • [21]Benatar JR, Jones E, White H, Stewart RA: A randomized trial evaluating the effects of change in dairy food consumption on cardio-metabolic risk factors. Eur J Prev Cardiol 2013. Epub ahead of print
  • [22]Diet History Questionnaire, Version 2.0. http://riskfactor.cancer.gov/dhq2/ webcite
  • [23]US Department of Agriculture [http://www.ers.usda.gov/ webcite]
  • [24]Brands of dairy products in New Zealand http://www.fonterrafoodservices.co.nz/brands.html?view=brand&id=17 webcite
  • [25]Beermann C, Jelinek J, Reinecker T, Hauenschild A, Boehm G, Klor H-U: Short term effects of dietary medium-chain fatty acids and n-3 long-chain polyunsaturated fatty acids on the fat metabolism of healthy volunteers. Lipids Health Dis 2003, 2:10. BioMed Central Full Text
  • [26]Vickers AJ, Altman DG: Analysing controlled trials with baseline and follow up measurements. BMJ 2001, 323:1123-1124.
  • [27]Kutner MH, Nachtsheim CJ, Neter J, Li W: Applied Linear Statistical Models. 5th edition. New York, NY: McGraw-Hill/Irwin; 2004.
  • [28]Proschan MA, Waclawiw MA: Practical guidelines for multiplicity adjustment in clinical trials. Control Clin Trials 2000, 21:527-539.
  • [29]Gebauer SK, Chardigny JM, Jakobsen MU, Lamarche B, Lock AL, Proctor SD, Baer DJ: Effects of ruminant trans fatty acids on cardiovascular disease and cancer: a comprehensive review of epidemiological, clinical, and mechanistic studies. Adv Nutr 2011, 2:332-354.
  • [30]Carroll SM, DePeters EJ, Taylor SJ, Rosenberg M, Perez-Monti H, Capps VA: Milk composition of Holstein, Jersey, and Brown Swiss cows in response to increasing levels of dietary fat. Anim Feed Sci Technol 2006, 131:451-473.
  • [31]Hu FB, Stampfer MJ, Manson JE, Ascherio A, Colditz GA, Speizer FE, Hennekens CH, Willett WC: Dietary saturated fats and their food sources in relation to the risk of coronary heart disease in women. Am J Clin Nutr 1999, 70:1001-1008.
  • [32]Hu FB, Manson JE, Willett WC: Types of dietary fat and risk of coronary heart disease: a critical review. J Am Coll Nutr 2001, 20:5-19.
  • [33]Aslibekyan S, Campos H, Baylin A: Biomarkers of dairy intake and the risk of heart disease. Nutr Metab Cardiovasc Dis 2012, 22:1039-1045.
  • [34]Nishida C, Uauy R: WHO Scientific Update on health consequences of trans fatty acids: introduction. Eur J Clin Nutr 2009, 63(Suppl 2):S1-S4.
  • [35]Mozaffarian D, Cao H, King IB, Lemaitre RN, Song X, Siscovick DS, Hotamisligil GS: Trans-palmitoleic acid, metabolic risk factors, and new-onset diabetes in U.S. Adults: a cohort study. Ann Int Med 2010, 153:790-799.
  • [36]Benatar JR, Gladding P, White HD, Zeng I, Stewart RA: Trans-fatty acids in New Zealand patients with coronary artery disease. Eur J Cardiovasc Prev Rehabil 2011, 18:615-620.
  • [37]Herzallah SM, Humeid MA, Al-Ismail KM: Effect of heating and processing methods of milk and dairy products on conjugated Linoleic acid and trans fatty acid Isomer content. J Dairy Sci 2005, 88:1301-1310.
  • [38]Roe M, Pinchen H, Church S, Elahi S, Walker M, Farron-Wilson M, Buttriss J, Finglas P: Trans fatty acids in a range of UK processed foods. Food Chem 2013, 140:427-431.
  • [39]Bhattacharya A, Banu J, Rahman M, Causey J, Fernandes G: Biological effects of conjugated linoleic acids in health and disease. J Nutr Biochem 2006, 17:789-810.
  • [40]Turpeinen AM, Mutanen M, Aro A, Salminen I, Basu S, Palmquist DL, Griinari JM: Bioconversion of vaccenic acid to conjugated linoleic acid in humans. Am J Clin Nutr 2002, 76:504-510.
  • [41]Wanders AJ, Brouwer IA, Siebelink E, Katan MB: Effect of a high intake of conjugated linoleic acid on lipoprotein levels in healthy human subjects. PLoS One 2010, 5:e9000.
  • [42]Chardigny JM, Destaillats F, Malpuech-Brugere C, Moulin J, Bauman DE, Lock AL, Barbano DM, Mensink RP, Bezelgues JB, Chaumont P, Combe N, Cristiani I, Joffre F, German JB, Dionisi F, Boirie Y, Sebedio J: Do trans fatty acids from industrially produced sources and from natural sources have the same effect on cardiovascular disease risk factors in healthy subjects? Results of the trans Fatty Acids Collaboration (TRANSFACT) study. Am J Clin Nutr 2008, 87:558-566.
  • [43]Schaefer EJ, Augustin JL, Schaefer MM, Rasmussen H, Ordovas JM, Dallal GE, Dwyer JT: Lack of efficacy of a food-frequency questionnaire in assessing dietary macronutrient intakes in subjects consuming diets of known composition. Am J Clin Nutr 2000, 71:746-751.
  • [44]Kristal AR, Peters U, Potter JD: Is it time to abandon the food frequency questionnaire? Cancer Epidemiol Biomarkers Prev 2005, 14:2826-2828.
  • [45]Subar AF, Thompson FE, Kipnis V, Midthune D, Hurwitz P, McNutt S, McIntosh A, Rosenfeld S: Comparative validation of the Block, Willett, and National Cancer Institute food frequency questionnaires : the Eating at America’s Table Study. Am J Epidemiol 2001, 154:1089-1099.
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