Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production

Virology Journal

Zhigao Bu²  Chinglai Yang¹  Dongni Kong²  Bolin Zhao²  Tao Hua²  Hongyue Zhai²  Zhiyuan Wen²  Xijun Wang²  Jinying Ge²  Lihong Tao² 

[1] Department of Microbiology and Immunology, Emory University School of Medicine, Rollins Research Center, Atlanta, GA 30322, USA;State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. China

关键词: inactivated vaccine;    recombinant;    LEP;    Rabies virus;   

Others  :  1156000
DOI  :  10.1186/1743-422X-8-454

 received in 2011-05-16, accepted in 2011-09-25,  发布年份 2011

【 摘 要 】

The rabies Flury Low Egg Passage virus (LEP) has been widely used as a seed virus to generate inactive vaccine. Here, we established a reverse genetic system for LEP and generated a recombinant LEP virus (rLEP-G) that carries two identical G genes. This recombinant virus showed similar properties to those of LEP with respect to in vitro growth, neurotropism index, and virulence in mice. rLEP-G produced 4.3-fold more G protein than did LEP in BHK-21 cells. The inactivated vaccine generated from rLEP-G induced significantly higher virus neutralization titers in mice and dogs than those produced in response to LEP-derived vaccine. Our results suggest that rLEP-G is an improved seed virus candidate for inactivated rabies virus vaccine manufacture.

【 授权许可】

   
2011 Tao et al; licensee BioMed Central Ltd.

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