Complete genome sequence of an astrovirus identified in a domestic rabbit (Oryctolagus cuniculus) with gastroenteritis

Virology Journal

Joseph L DeRisi²  Melissa A Kennedy¹  Donald Ganem²  Julia S Lankton¹  J Graham Ruby⁴  Rebecca P Wilkes¹  Cheryl Greenacre³  Eric Velazquez⁴  Mark D Stenglein⁴ 

[1] Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, USA;Howard Hughes Medical Institute, Chevy Chase, MD, USA;Department of Small Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, USA;Departments of Medicine, Biochemistry and Biophysics, and Microbiology, University of California San Francisco, San Francisco, CA, USA

关键词: Enterocolitis;    Mucoid enteropathy;    Diarrhea virus;    Gastroenteritis;    Virochip;    Virus discovery;    Rabbit virus;    Rabbit astrovirus;   

Others  :  1153708
DOI  :  10.1186/1743-422X-9-216

 received in 2012-01-06, accepted in 2012-09-14,  发布年份 2012

【 摘 要 】

A colony of domestic rabbits in Tennessee, USA, experienced a high-mortality (~90%) outbreak of enterocolitis. The clinical characteristics were one to six days of lethargy, bloating, and diarrhea, followed by death. Heavy intestinal coccidial load was a consistent finding as was mucoid enteropathy with cecal impaction. Preliminary analysis by electron microscopy revealed the presence of virus-like particles in the stool of one of the affected rabbits. Analysis using the Virochip, a viral detection microarray, suggested the presence of an astrovirus, and follow-up PCR and sequence determination revealed a previously uncharacterized member of that family. Metagenomic sequencing enabled the recovery of the complete viral genome, which contains the characteristic attributes of astrovirus genomes. Attempts to propagate the virus in tissue culture have yet to succeed. Although astroviruses cause gastroenteric disease in other mammals, the pathogenicity of this virus and the relationship to this outbreak remains to be determined. This study therefore defines a viral species and a potential rabbit pathogen.

【 授权许可】

   
2012 Stenglein et al.; licensee BioMed Central Ltd.

【 图 表 】

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