In vitro inhibition of monkeypox virus production and spread by Interferon-β

Virology Journal

Lisa E Hensley²  Arthur Goff²  Gordon Ruthel¹  John H Connor³  Kenny L Lin²  Sara C Johnston² 

[1]Toxicology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St. Fort Detrick, Frederick, MD 21702, USA

[2]Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St. Fort Detrick, Frederick, MD 21702, USA

[3]Microbiology Department, Boston University School of Medicine and Microbiology, 72 E.Concord St R-Bd Boston, Boston, MA 02118, USA

关键词: MxA;    IFN-β;    Type I interferon;    Monkeypox virus;    Orthopoxvirus;   

Others  :  1155311
DOI  :  10.1186/1743-422X-9-5

 received in 2011-12-07, accepted in 2012-01-06,  发布年份 2012

【 摘 要 】

Background

The Orthopoxvirus genus contains numerous virus species that are capable of causing disease in humans, including variola virus (the etiological agent of smallpox), monkeypox virus, cowpox virus, and vaccinia virus (the prototypical member of the genus). Monkeypox is a zoonotic disease that is endemic in the Democratic Republic of the Congo and is characterized by systemic lesion development and prominent lymphadenopathy. Like variola virus, monkeypox virus is a high priority pathogen for therapeutic development due to its potential to cause serious disease with significant health impacts after zoonotic, accidental, or deliberate introduction into a naïve population.

Results

The purpose of this study was to investigate the prophylactic and therapeutic potential of interferon-β (IFN-β) for use against monkeypox virus. We found that treatment with human IFN-β results in a significant decrease in monkeypox virus production and spread in vitro. IFN-β substantially inhibited monkeypox virus when introduced 6-8 h post infection, revealing its potential for use as a therapeutic. IFN-β induced the expression of the antiviral protein MxA in infected cells, and constitutive expression of MxA was shown to inhibit monkeypox virus infection.

Conclusions

Our results demonstrate the successful inhibition of monkeypox virus using human IFN-β and suggest that IFN-β could potentially serve as a novel safe therapeutic for human monkeypox disease.

【 授权许可】

   
2011 Johnston et al; licensee BioMed Central Ltd.

【 图 表 】

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