ABC transporters are involved in defense against permethrin insecticide in the malaria vector Anopheles stephensi

Particle and Fibre Toxicology

Sandra Urbanelli²  Claudio Bandi⁵  Claudio Genchi⁵  Guido Favia¹  Domenico Otranto³  Claudia Cafarchia³  Paolo Rossi¹  Davide Sassera⁴  Francesco Comandatore⁴  Valentina Mastrantonio²  Daniele Porretta²  Sara Epis⁵ 

[1] School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy;Department of Environmental Biology, University “La Sapienza” of Rome, Rome, Italy;Department of Veterinary Medicine, University of Bari, Bari, Italy;Department of Biology and Biotechnology, University of Pavia, Pavia, Italy;Department of Veterinary Science and Public Health, University of Milan, Milan, Italy

关键词: ABC transporters;    Vector control;    Culicidae;    Insecticide resistance;    Bioassays;    Mosquitoes;   

Others  :  1181887
DOI  :  10.1186/1756-3305-7-349

 received in 2014-06-05, accepted in 2014-07-15,  发布年份 2014

【 摘 要 】

Background

Proteins from the ABC family (ATP-binding cassette) represent the largest known group of efflux pumps, responsible for transporting specific molecules across lipid membranes in both prokaryotic and eukaryotic organisms. In arthropods they have been shown to play a role in insecticide defense/resistance. The presence of ABC transporters and their possible association with insecticide transport have not yet been investigated in the mosquito Anopheles stephensi, the major vector of human malaria in the Middle East and South Asian regions. Here we investigated the presence and role of ABCs in transport of permethrin insecticide in a susceptible strain of this mosquito species.

Methods

To identify ABC transporter genes we obtained a transcriptome from untreated larvae of An. stephensi and then compared it with the annotated transcriptome of Anopheles gambiae. To analyse the association between ABC transporters and permethrin we conducted bioassays with permethrin alone and in combination with an ABC inhibitor, and then we investigated expression profiles of the identified genes in larvae exposed to permethrin.

Results

Bioassays showed an increased mortality of mosquitoes when permethrin was used in combination with the ABC-transporter inhibitor. Genes for ABC transporters were detected in the transcriptome, and five were selected (AnstABCB2, AnstABCB3, AnstABCB4, AnstABCmember6 and AnstABCG4). An increased expression in one of them (AnstABCG4) was observed in larvae exposed to the LD50 dose of permethrin. Contrary to what was found in other insect species, no up-regulation was observed in the AnstABCB genes.

Conclusions

Our results show for the first time the involvement of ABC transporters in larval defense against permethrin in An. stephensi and, more in general, confirm the role of ABC transporters in insecticide defense. The differences observed with previous studies highlight the need of further research as, despite the growing number of studies on ABC transporters in insects, the heterogeneity of the results available at present does not allow us to infer general trends in ABC transporter-insecticide interactions.

【 授权许可】

   
2014 Epis et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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