Insecticide susceptibility and dengue vector status of wild Stegomyia albopicta in a strategically important area of Assam, India

Particle and Fibre Toxicology

Vijay veer¹  Indra Baruah¹  Kavita Yadav¹  Bipul Rabha¹  Sunil Dhiman¹ 

[1] Defence Research Laboratory, Tezpur Assam-784 001, India

关键词: VectorTest™ assay;    Insecticide resistance;    Northeast India;    Dengue;    St. albopicta;   

Others  :  802400
DOI  :  10.1186/1756-3305-7-295

 received in 2014-02-27, accepted in 2014-06-26,  发布年份 2014

【 摘 要 】

Background

Dengue vector control programmes are facing operational challenges due to resistance against commonly used insecticides throughout the endemic countries. Recently, there has been appreciable increase in the dengue cases in India, however, no recent data are available on susceptible status of dengue vectors. We have studied the susceptibility level of St. albopicta to commonly used insecticides in India. Adult mosquitoes were tested for the presence of dengue virus.

Methods

St. albopicta larval bioassays were carried out to determine the lethal concentrations (LC₁₀, LC₅₀ and LC₉₉) and the resistance ratios (RR₁₀, RR₅₀ and RR₉₉) for temephos. Susceptibility to 4% DDT, 0.05% deltamethrin and 5% malathion was assessed following standard procedure. Knock-down times (KDT₁₀, KDT₅₀ and KDT₉₉) were estimated and knock-down resistance ratios (KRR₁₀, KRR₅₀ and KRR₉₉) were calculated. VectorTest™ dengue antigen assay was used to detect the dengue virus in the field collected mosquitoes.

Results

In larval bioassays, the RR ranged from 1.4 (for RR₉₉) to 1.7 (for RR₅₀), which suggested that the tested St. albopicta were susceptible to temephos. There was no deviation among the lethal concentration data from linearity (r² = 0.61). Adult St. albopicta mosquitoes were resistant to DDT, while fully susceptible to deltamethrin and malathion. The knock-down values (KDT₁₀, KDT₅₀ and KDT₉₉) obtained for DDT displayed straight line in log-dose-probit analysis and follow linear regression model. The KRR₉₉ for DDT was 4.9, which indicated a 4.9 folds increase in knock-down resistance to DDT. However, for malathion and deltamethrin, the KRR₉₉ values were 1.6 and 1.5 respectively suggesting that mosquitoes were knock-down sensitive. None of the mosquito pool was dengue virus positive.

Conclusion

St. albopicta showed resistance to DDT and reduced sensitivity to deltamethrin and malathion. This data on insecticide resistance could help public health authorities in India to design more effective vector control measures. More dengue vector specimens need to be scanned to identify the potential dengue vector.

【 授权许可】

   
2014 Dhiman et al.; licensee BioMed Central Ltd.

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