Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture

Respiratory Research

Stephen I Rennard³  Ronald F Ertl³  Fu-Qiang Wen³  Tadashi Kohyama³  Hangjun Wang¹  Xiangde Liu³  C Magnus Sköld²  Yunkui Zhu³ 

[1] Mount Sinai Hospital, Pathology and Laboratory Medicine, Toronto, Ontario, Canada;Karolinska Hospital, Stockholm, Sweden;University of Nebraska Medical Center, Omaha, Nebraska, USA

关键词: TNF-α;    PGE₂;    neutrophil elastase;    monocytes;    lung fibroblasts;    IL-1;    Collagen degradation;   

Others  :  1227427
DOI  :  10.1186/rr72

 received in 2001-03-05, accepted in 2001-08-08,  发布年份 2001

【 摘 要 】

Background

Inflammatory cells are believed to play a prominent role during tissue repair and remodeling. Since repair processes develop and mature over extended time frames, the present study was designed to evaluate the effect of monocytes and fibroblasts in prolonged culture in three-dimensional collagen gels.

Methods

Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks.

Results

Fibroblast-mediated gel contraction was initially inhibited by the presence of monocytes (P < 0.01). However, with extended co-culture, contraction of the collagen gels was greatly augmented (P < 0.01). In addition, with extended co-culture, degradation of collagen in the gels occurred. The addition of neutrophil elastase to the medium augmented both contraction and degradation (P < 0.01). Prostaglandin E₂ production was significantly increased by co-culture and its presence attenuated collagen degradation.

Conclusion

The current study, therefore, demonstrates that interaction between monocytes and fibroblasts can contract and degrade extracellular matrix in extended culture.

【 授权许可】

   
2001 Zhu etal, licensee BioMed Central Ltd

【 图 表 】

Figure 1.

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Supplementary Figure 5.

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