Prevalence of classic, MLB-clade and VA-clade Astroviruses in Kenya and The Gambia

Virology Journal

Lori R. Holtz³  David Wang³  O. Colin Stine¹  Richard Omore⁴  John B. Ochieng⁴  Joseph O. Oundo⁴  Debasish Saha²  Mitchell Adeyemi⁵  Martin Antonio⁵  Irma K. Bauer³  Caroline T. Meyer³ 

[1] University of Maryland School of Medicine, Baltimore, MD, USA;Center for International Health, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand;Washington University School of Medicine, Saint Louis, MO, USA;Kenya Medical Research Institute/Centers for Disease Control and Prevention, Kisumu, Kenya;Medical Research Council Unit, Banjul, The Gambia

关键词: Gastroenteritis;    Diarrhea;    VA;    MLB;    Astrovirus;   

Others  :  1224935
DOI  :  10.1186/s12985-015-0299-z

 received in 2015-01-23, accepted in 2015-04-15,  发布年份 2015

【 摘 要 】

Background

Infectious diarrhea leads to significant mortality in children, with 40 % of these deaths occurring in Africa. Classic human astroviruses are a well-established etiology of diarrhea. In recent years, seven novel astroviruses have been discovered (MLB1, MLB2, MLB3, VA1/HMO-C, VA2/HMO-B, VA3/HMO-A, VA4); however, there have been few studies on their prevalence or potential association with diarrhea.

Methods

To investigate the prevalence and diversity of these classic and recently described astroviruses in a pediatric population, a case–control study was performed. Nine hundred and forty nine stools were previously collected from cases of moderate-to-severe diarrhea and matched controls of patients less than 5 years of age in Kenya and The Gambia. RT-PCR screening was performed using pan-astrovirus primers.

Results

Astroviruses were present in 9.9 % of all stool samples. MLB3 was the most common astrovirus with a prevalence of 2.6 %. Two subtypes of MLB3 were detected that varied based on location in Africa. In this case–control study, Astrovirus MLB1 was associated with diarrhea in Kenya, whereas Astrovirus MLB3 was associated with the control state in The Gambia. Classic human astrovirus was not associated with diarrhea in this study. Unexpectedly, astroviruses with high similarity to Canine Astrovirus and Avian Nephritis Virus 1 and 2 were also found in one case of diarrhea and two control stools respectively.

Conclusions

Astroviruses including novel MLB- and VA-clade members are commonly found in pediatric stools in Kenya and The Gambia. The most recently discovered astrovirus, MLB3, was the most prevalent and was found more commonly in control stools in The Gambia, while astrovirus MLB1 was associated with diarrhea in Kenya. Furthermore, a distinct subtype of MLB3 was noted, as well as 3 unanticipated avian or canine astroviruses in the human stool samples. As a result of a broadly reactive PCR screen for astroviruses, new insight was gained regarding the epidemiology of astroviruses in Africa, where a large proportion of diarrheal morbidity and mortality occur.

【 授权许可】

   
2015 Meyer et al.

【 图 表 】

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