Vitamin A deficiency alters the pulmonary parenchymal elastic modulus and elastic fiber concentration in rats

Respiratory Research

Amey J Holmes¹  Erika J Takle¹  Stephen E McGowan¹ 

[1] Department of Veterans Affairs Research Service and Department of Internal Medicine, Roy A. and Lucille J Carver College of Medicine, University of Iowa, Iowa City, IA, USA

关键词: cholinergic;    bronchial hyperreactivity;    emphysema;    retinoic acid;    Elastin;   

Others  :  1227268
DOI  :  10.1186/1465-9921-6-77

 received in 2005-02-01, accepted in 2005-07-20,  发布年份 2005

【 摘 要 】

Background

Bronchial hyperreactivity is influenced by properties of the conducting airways and the surrounding pulmonary parenchyma, which is tethered to the conducting airways. Vitamin A deficiency (VAD) is associated with an increase in airway hyperreactivity in rats and a decrease in the volume density of alveoli and alveolar ducts. To better define the effects of VAD on the mechanical properties of the pulmonary parenchyma, we have studied the elastic modulus, elastic fibers and elastin gene-expression in rats with VAD, which were supplemented with retinoic acid (RA) or remained unsupplemented.

Methods

Parenchymal mechanics were assessed before and after the administration of carbamylcholine (CCh) by determining the bulk and shear moduli of lungs that that had been removed from rats which were vitamin A deficient or received a control diet. Elastin mRNA and insoluble elastin were quantified and elastic fibers were enumerated using morphometric methods. Additional morphometric studies were performed to assess airway contraction and alveolar distortion.

Results

VAD produced an approximately 2-fold augmentation in the CCh-mediated increase of the bulk modulus and a significant dampening of the increase in shear modulus after CCh, compared to vitamin A sufficient (VAS) rats. RA-supplementation for up to 21 days did not reverse the effects of VAD on the elastic modulus. VAD was also associated with a decrease in the concentration of parenchymal elastic fibers, which was restored and was accompanied by an increase in tropoelastin mRNA after 12 days of RA-treatment. Lung elastin, which was resistant to 0.1 N NaOH at 98°, decreased in VAD and was not restored after 21 days of RA-treatment.

Conclusion

Alterations in parenchymal mechanics and structure contribute to bronchial hyperreactivity in VAD but they are not reversed by RA-treatment, in contrast to the VAD-related alterations in the airways.

【 授权许可】

   
2005 McGowan et al; licensee BioMed Central Ltd.

【 图 表 】

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