Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer

Virology Journal

Xi Li¹  Lihua Hu²  Ying Liu²  Xueyun Zhang¹  Fang Fu¹  Peiyi Zhang²  Zheng Chai¹ 

[1] State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan St., Nangang District, Harbin 150001, China;Gastrointestinal Department of Heilongjiang Province Hospital, Harbin 150000, China

关键词: Nude mice;    Recombinant virus;    Oncolytic therapy;    Lung cancer;    Newcastle disease virus;   

Others  :  804562
DOI  :  10.1186/1743-422X-11-84

 received in 2014-01-28, accepted in 2014-04-30,  发布年份 2014

【 摘 要 】

Background

Lung cancer is one of the leading causes of deaths from cancer worldwide. Tumor virotherapy using naturally oncolytic Newcastle disease virus (NDV) has been shown to be safe and effective in preclinical studies and clinical trials. Previously, we have reported the NDV D90 strain that was isolated from natural source has an antiproliferative effect in human lung cancer cell line A549.

Methods and results

In this study, we constructed a reverse genetics system based on the oncolytic NDV D90 strain and generated a recombinant NDV carrying a gene encoding enhanced green fluorescent protein (rNDV-GFP). The rescued virus rNDV-D90 and rNDV-GFP showed the similar characteristics of replication and apoptotic ability in lung cancer A549 cells, which suggested that the recombinant viruses sustained the property of tumor-selective replication and induced apoptosis of tumor cells. The athymic mice bearing implanted lung cancer were treated with the parental D90 virus, the rescued rNDV-D90 and rNDV-GFP via intratumoral injections, respectively. The results showed that the recombinant viruses as well as the parental D90 virus significantly suppressed the loss of body weight and tumor growth.

Conclusions

The study provides a new platform to develop effective therapeutic agents for tumor treatment. The availability of the reverse genetics system for NDV D90 strain will make it possible to develop novel recombinant oncolytic viruses based on the NDV D90 strain for improving the efficacy of tumor treatment.

【 授权许可】

   
2014 Chai et al.; licensee BioMed Central Ltd.

【 图 表 】

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