Molecular evidence of Ebola Reston virus infection in Philippine bats

Virology Journal

Scott HNewman⁵  Peter Daszak⁶  Lin-Fa Wang¹  Jonathan HEpstein⁶  Carolyn CBenigno³  Theresa Mundita S. Lim⁹  Imelda JSantos⁸  Magdalena SCruz^1,10  Meng Yu^1,10  Adam Foord⁴  Lilia MRetes^1,10  Rachel RAzul^1,10  Jennifer Barr⁴  Anthony CBucad^1,10  Tom Hughes⁶  Anson MTagtag⁹  Glenn Marsh⁴  Kevin JOlival⁶  Carol de Jong⁷  Hume EField⁶  Sarah IJayme² 

[1]Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, ᅟ, Singapore

[2]Global Alliance for Rabies Control, Santa Rosa City, Philippines

[3]Food and Agriculture Organization of the United Nations Regional Office for Asia and the Pacific (FAO RAP), Bangkok, Thailand

[4]CSIRO Australian Animal Health Laboratory, Geelong, Australia

[5]Food and Agriculture Organization of the United Nations, Emergency Centre for Transboundary Animal Disease, Hanoi, Vietnam

[6]EcoHealth Alliance, New York, USA

[7]Queensland Centre for Emerging Infectious Diseases, Department of Agriculture, Fisheries and Forestry, Brisbane, Australia

[8]Food and Agriculture Organization of the United Nations, Makati City, Philippines

[9]Biodiversity Management Bureau, Department of Environment and Natural Resources, Quezon City, Philippines

[10]Bureau of Animal Industries, Department of Agriculture, Quezon City, Philippines

关键词: Serology;    Molecular;    Bat;    Philippine;    Filovirus;    Ebolavirus;    Reston;   

Others  :  1224901
DOI  :  10.1186/s12985-015-0331-3

 received in 2015-06-12, accepted in 2015-06-26,  发布年份 2015

【 摘 要 】

Background

In 2008–09, evidence of Reston ebolavirus (RESTV) infection was found in domestic pigs and pig workers in the Philippines. With species of bats having been shown to be the cryptic reservoir of filoviruses elsewhere, the Philippine government, in conjunction with the Food and Agriculture Organization of the United Nations, assembled a multi-disciplinary and multi-institutional team to investigate Philippine bats as the possible reservoir of RESTV.

Methods

The team undertook surveillance of bat populations at multiple locations during 2010 using both serology and molecular assays.

Results

A total of 464 bats from 21 species were sampled. We found both molecular and serologic evidence of RESTV infection in multiple bat species. RNA was detected with quantitative PCR (qPCR) in oropharyngeal swabs taken from Miniopterus schreibersii, with three samples yielding a product on conventional hemi-nested PCR whose sequences differed from a Philippine pig isolate by a single nucleotide. Uncorroborated qPCR detections may indicate RESTV nucleic acid in several additional bat species (M. australis, C. brachyotis and Ch. plicata). We also detected anti-RESTV antibodies in three bats (Acerodon jubatus) using both Western blot and ELISA.

Conclusions

The findings suggest that ebolavirus infection is taxonomically widespread in Philippine bats, but the evident low prevalence and low viral load warrants expanded surveillance to elaborate the findings, and more broadly, to determine the taxonomic and geographic occurrence of ebolaviruses in bats in the region.

【 授权许可】

   
2015 Food and Agriculture Organization of the United Nations

【 图 表 】

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