Efficacy of indoxacarb applied to cats against the adult cat flea, Ctenocephalides felis, flea eggs and adult flea emergence

Particle and Fibre Toxicology

Fangshi Sun¹  Kathleen Heaney¹  Vicki Smith²  Patricia A Payne²  Michael W Dryden² 

[1]Merck Animal Health, 556 Morris Avenue, Summit, NJ, 07901, USA

[2]Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, 66506, USA

关键词: Efficacy;    Adulticidal activity;    Egg production;    Indoxacarb;    Cats;    Cat flea;    Ctenocephalides felis felis;    Flea;   

Others  :  1227970
DOI  :  10.1186/1756-3305-6-126

 received in 2013-04-05, accepted in 2013-04-26,  发布年份 2013

【 摘 要 】

Background

A study was conducted to evaluate the effect of indoxacarb applied to cats on adult cat fleas, Ctenocephalides felis, flea egg production and adult flea emergence.

Methods

Sixteen cats were selected for the study and allocated to two treatment groups. Eight cats were treated with a 19.5% w/v topical spot-on solution of indoxacarb on day 0 and eight cats served as untreated controls. Each cat was infested with 50 fleas on Days -2, 7, 14, 21, 28, 35 and 42. On Days 1, 2, and 3, and at 2 and 3 days after each post treatment reinfestation flea eggs were collected from the pan under each cat cage. Eggs were counted and viability assessed by evaluating adult flea emergence 28 days after egg collection. Three days after treatment or infestation, each cat was combed to remove and count live fleas.

Results

Treatment with indoxacarb provided 100% efficacy following infestations on day -2, 7, 14, 21 and 28 and efficacy was 99.6% following infestations on days 35 and 42. Egg production from indoxacarb treated cats was reduced by 99.9% within 72 hours of treatment. For subsequent infestations no eggs were produced from treated cats from day 8 through day 30. Egg production was still reduced by ≥95.8% through day 45. Indoxacarb treatment also reduced adult flea emergence from eggs for 5 weeks after treatment. The combination of reduction in egg numbers and egg viability from indoxacarb treated cats reduced predicted flea emergence by 100% from days 2 – 31 and 99.9%, 100%, 96.4% and 99.0% on days 37, 38, 44 and 45, respectively.

Conclusions

A topical spot-on formulation of indoxacarb provided ≥99.6% efficacy against flea infestations on cats for 6 weeks following a single treatment. Indoxacarb also eliminated or markedly reduced egg production for the entire evaluation period and reduced the viability of the few eggs that were produced from Day 1 through Day 38. Given indoxacarb’s effect on adult fleas, egg production and egg viability; this formulation can interrupt flea reproduction on treated cats for at least 6 weeks after treatment.

【 授权许可】

   
2013 Dryden et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Blagburn BL, Dryden MW: Biology, treatment and control of flea and tick infestations. Vet Clin N Am 2009, 39(6):1173-1200.
• [2]Akin DE: Relationship between feeding and reproduction in the cat flea, Ctenocephalides felis (Bouche). M.S. Thesis: University of Florida, Department of Entomology; 1984.
• [3]Dryden MW: Host association, on host longevity and Egg production of ctenocephalides felis. Vet Parasitol 1989, 34:117-122.
• [4]Dryden MW, Broce AB: Integrated flea control for the 21st Century. Comp Cont Ed Pract Vet 2002, 24((1) suppl):36-39.
• [5]Rust MK: Advances in the control of Ctenocephalides felis felis (cat flea) on cats and dogs. Trends Parasitol 2005, 21:232-236.
• [6]Chin A, Lunn P, Dryden MW: Persistent flea infestations in dogs and cats controlled with monthly topical applications of fipronil and methoprene. Aust Vet Pract 2005, 35(3):89-96.
• [7]Dryden MW: Flea and tick control in the 21st century, challenges and opportunities. Vet Dermatol 2009, 20:435-440.
• [8]Dryden MW, Perez HR, Ulitchny DM: Control of flea populations on naturally infested dogs and cats and in private residences with either topical imidacloprid spot application or the combination of oral lufenuron and pyrethrin spray. Am J Vet Med Assoc 1999, 215(1):36-39.
• [9]Dryden MM, Magid-Denenberg T, Bunch S: Control of fleas on naturally infested dogs and cats and in private residences with topical spot applications of fipronil or imidacloprid. Vet Parasitol 2000, 93:69-75.
• [10]Robertson-Plouch C, Baker KA, Hozak RR, Zimmermann AG, Parks SC, Herr C, Hart LM, Jay J, Hutchens DE, Snyder DE: Clinical field study of the safety and efficacy of spinosad chewable tablets for controlling fleas on dogs. Vet Therapeutics 2008, 9:26-36.
• [11]Beugnet F, Franc M: Results of a European multicentric field efficacy study of fipronil-(S) methoprene combination on flea infestation of dogs and cats during 2009 summer. Parasite 2010, 17(4):337-342.
• [12]Dryden MW, Payne PA, Smith V, Riggs B, Davenport J, Kobuszewski D: Efficacy of dinotefuran–pyriproxyfen, dinotefuran–pyriproxyfen–permethrin and fipronil–(S)-methoprenetopical spot-on formulations to control flea populations in naturally infested pets and private residences in Tampa, FL. Vet Parasitol 2011, 182:281-286.
• [13]Dryden MW, Ryan WG, Bell M, Rumschlag AJ, Young LM, Snyder DE: Assessment of owner-administered monthly treatments with oral spinosad or topical spot-on fipronil/(S)-methoprene in controlling fleas and associated pruritus in dogs. Vet Parasitol 2013, 191:340-346.
• [14]McCann SF, Cordova D, Andaloro JT, Lahm GP: Sodium channel blocking insecticides, indoxacarb. In Modern crop protection compounds. Volume 1. Edited by Kramer W, Schirmer U. Wenheim: Wiley-VCH; 2007:1031-1048.
• [15]Wing KD, Andaloro JT, McCann SF, Salgado VL: Indoxacarb and the sodium channel blocker insecticides: chemistry, physiology and biology in insects. In Insect control biological and synthetic agents. Edited by Gilbert LI, Gill SS. London: Elsevier, B.V; 2010:35-57.
• [16]Wing KD, Sacher M, Kagaya Y, Tsurubuchi Y, Mulderig L, Connair M, Schnee M: Bioactivation and mode of action of the oxadiazine indoxacarb in insects. Crop Prot 2000, 19:537-545.
• [17]Silver KS, Song W, Nomura Y, Salgado VL, Dong K: Mechanism of action of sodium channel blocker insecticides (SCBIs) on insect sodium channels. Pest Biochem Phys 2010, 97:87-92.
• [18]Zhao X, Ikeda T, Yeh TZ, Narahashi T: Voltage-dependent block of sodium channels in mammalian neurons by the oxadiazine insecticide indoxacarb and its metabolite DCJW. NeuroToxicol 2003, 24:83-96.
• [19]Dryden M, Payne P, Smith V: Efficacy of selamectin and fipronil ⁄ (S)-methoprene spot-on formulations applied to dogs against the adult cat flea, Ctenocephalides felis, flea eggs and adult flea emergence. Vet Therapeutics 2007, 8:255-262.
• [20]Payne PA, Dryden MW, Smith V, Ridley RK: Effect of 0.29% w/w fipronil spray on adult flea mortality and egg production of three different cat flea, Ctenocephalides felis (Bouché), strains infesting dogs. Vet Parasitol 2001, 102(4):331-340.
• [21]Bossard RL, Dryden MW, Broce AB: Insecticide susceptibilities of cat fleas (Siphonaptera: Pulicidae) from several regions of the United States. J Med Entomol 2002, 39:742-746.
• [22]Rust MK, Waggoner M, Hinkle NC, Mencke N, Hansen O, Vaughn M, Dryden MW, Payne P, Blagburn B, Jacobs DE, Bach T, Bledsoe D, Hopkins T, Mehlhorn H: Development of a larval bioassay for susceptibility of cat fleas (Siphonaptera: Pulicidae) to imidacloprid. J Med Entomol 2002, 39:671-674.
• [23]Bass C, Schroeder I, Turberg A, Field L, Williamson MS: Identification of mutations associated with pyrethroid resistance in the para-type sodium channel of the cat flea, Ctenocephalides felis. Insect Biochem Mol Bio 2004, 34:1305-1313.
• [24]Dryden MW, Payne PA, Smith V, Kobuszewski D: Efficacy of topically applied dinotefuran formulations and orally administered spinosad tablets against the KS1 flea strain infesting dogs. Intern J Appl Research Vet Med 2010, 9(2):123-128.