Vitamin C and Vitamin E in Prevention of Nonalcoholic Fatty Liver Disease (NAFLD) in Choline Deficient Diet Fed Rats

Nutrition Journal

Flair José Carrilho⁴  Francisco RM Laurindo³  Fabio P Lopasso⁴  Dulcinéia SP Abdalla²  Emerson S Lima²  Bianca Ishimoto Della Nina⁴  Caroline Tatai⁴  Luiz Carlos da Costa Gayotto¹  Claudia PMS Oliveira⁴ 

[1] Department of Pathology (LIM14) of Medical School of University of São Paulo (USP), São Paulo, Brazil;Department of Clinical and Toxicological Analyses of School of Pharmaceutical Sciences of University of São Paulo (USP), São Paulo, Brazil;Departament of Heart Institute of Medical School of University of São Paulo (USP), São Paulo, Brazil;Department of Gastroenterology of Medical School of University of São Paulo (USP), São Paulo, Brazil

关键词: oxidative stress;    nonalcoholic fatty liver disease;    vitamin E;    vitamin C;   

Others  :  1152154
DOI  :  10.1186/1475-2891-2-9

 received in 2003-07-20, accepted in 2003-10-07,  发布年份 2003

【 摘 要 】

Aim

Oxidative stress has been implicated in the pathogenesis of Nonalcoholic Fatty Liver Disease (NAFLD). Vitamin C and vitamin E are known to react with reactive oxygen species (ROS) blocking the propagation of radical reactions in a wide range of oxidative stress situations. The potential therapeutic efficacy of antioxidants in NAFLD is unknown. The aim of this study was to evaluate the role of antioxidant drugs (vitamin C or vitamin E) in its prevention.

Methods

Fatty liver disease was induced in Wistar rats by choline-deficient diet for four weeks. The rats were randomly assigned to receive vitamin E (n = 6) – (200 mg/day), vitamin C (n = 6) (30 mg/Kg/day) or vehicle orally.

Results

In the vehicle and vitamin E-treated rats, there were moderate macro and microvesicular fatty changes in periportal area without inflammatory infiltrate or fibrosis. Scharlach stain that used for a more precise identification of fatty change was strong positive. With vitamin C, there was marked decrease in histological alterations. Essentially, there was no liver steatosis, only hepatocellular ballooning. Scharlach stain was negative. The lucigenin-enhanced luminescence was reduced with vitamin C (1080 ± 330 cpm/mg/minx10³) as compared to those Vitamin E and control (2247 ± 790; 2020 ± 407 cpm/mg/minx10³, respectively) (p < 0.05). Serum levels of aminotransferases were unaltered by vitamin C or vitamin E.

Conclusions

1) Vitamin C reduced oxidative stress and markedly inhibited the development of experimental liver steatosis induced by choline-deficient diet ; 2)Vitamin E neither prevented the development of fatty liver nor reduced the oxidative stress in this model.

【 授权许可】

   
2003 Oliveira et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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