RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity

Particle and Fibre Toxicology

Hong Yin¹  Jianxun Luo¹  Youquan Li¹  Zhijie Liu¹  Guiquan Guan¹  Qiong Wu¹ 

[1] State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Grazing Animal Diseases MOA, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China

关键词: Transcriptional profile;    High-throughput sequencing;    Gene expression;    Lyme disease;   

Others  :  1146654
DOI  :  10.1186/s13071-014-0623-2

 received in 2014-09-27, accepted in 2014-12-26,  发布年份 2015

【 摘 要 】

Background

Lyme disease is a global public health problem caused by the spirochaete Borrelia burgdorferi. Our previous studies found differences in disease severity between B. burgdorferi B31- and B. garinii SZ-infected mice. We hypothesized that genes that are differentially expressed between Borrelia isolates encode bacterial factors that contribute to disease diversity.

Methods

The present study used high-throughput sequencing technology to characterize and compare the transcriptional profiles of B. burgdorferi B31 and B. garinii SZ cultured in vitro. Real-time quantitative RT-PCR was used to validate selected data from RNA-seq experiments.

Results

A total of 731 genes were differentially expressed between B. burgdorferi B31 and B. garinii SZ isolates, including those encoding lipoproteins and purine transport proteins. The fold difference in expression for B. garinii SZ versus B. burgdorferi B31 ranged from 22.07 to 1.01. Expression of the OspA, OspB and DbpB genes were significantly lower in B. garinii SZ compared to B. burgdorferi B31.

Conclusions

The results support the hypothesis that global changes in gene expression underlie differences in Borrelia pathogenicity. The findings also provide an empirical basis for studying the mechanism of action of specific genes as well as their potential usefulness for the diagnosis and management of Lyme disease.

【 授权许可】

   
2015 Wu et al.; licensee BioMed Central.

【 图 表 】

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