Prevention of Babesia canis in dogs: efficacy of a fixed combination of permethrin and fipronil (Effitix®) using an experimental transmission blocking model with infected Dermacentor reticulatus ticks

Particle and Fibre Toxicology

Stéphane Bonneau¹  Klaus Hellmann²  Josephus Fourie³  Nadège Reymond⁴  Christelle Navarro¹ 

[1] Virbac, 13ième rue - LID, Carros, 06511, France;KLIFOVET AG, Geyerspergerstr. 27, Munich, 80689, Germany;ClinVet International, Universitas, 9321, South Africa;Nadege Savelli EIRL, 2, rue de l’église, Villeneuve Loubet, 06 270, France

关键词: Transmission blocking;    Permethrin;    Fipronil;    Ectoparasiticides;    Ticks;    Dermacentor reticulatus;    Babesia canis;   

Others  :  1147783
DOI  :  10.1186/s13071-015-0645-4

 received in 2014-11-10, accepted in 2015-01-05,  发布年份 2015

【 摘 要 】

Background

Two experimental studies using a transmission blocking model with Dermacentor reticulatus ticks infected with Babesia canis were performed to test the ability of Effitix® to prevent the transmission of babesiosis in dogs.

Methods

Four groups of seven dogs (experiment 1) and one group of eight dogs (experiment 2) were treated topically with a novel combination of fipronil and permethrin in a spot-on formulation (Effitix®, Virbac) respectively 28, 21, 14 and 7 days (experiment 1) and 2 days (experiment 2) prior to tick infestation. In each study, a control group of seven dogs (experiment 1) and eight dogs (experiment 2) remained untreated. On day 0, all dogs were infested with adult D.reticulatus ticks harboring B. canis. An efficacy failure (successfully infected) was regarded as a dog in the treated groups that was tested serologically positive for B.canis antibodies, using an indirect fluorescent antibody (IFA) assay and tested positive for B.canis by DNA-assay using PCR analysis.

Results

B.canis was transmitted by D.reticulatus to all untreated dogs (experiment 1) and six untreated dogs out of eight (experiment 2) as confirmed by IFA and PCR assays. The large majority of treated dogs (92.9% in experiment 1 and 100% in experiment 2) remained sero-negative over the challenge period.

Conclusions

The treatment of dogs with Effitix® applied 2 to 28 days prior to infestation with D. reticulatus harboring B.canis, successfully prevented the transmission of canine babesiosis.

【 授权许可】

   
2015 Navarro et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]René-Martellet M, Chêne J, Chabanne L, Chalvet-Monfray K, Bourdoiseau G. Clinical signs, seasonal occurrence and causative agents of canine babesiosis in France: results of a multiregional study. Vet Parasitol. 2013; 197:50-8.
• [2]Kubelová M, Tkadlec E, Bednář M, Roubalová E, Siroký P. West-to-east differences of Babesia canis canis prevalence in Dermacentor reticulatus ticks in Slovakia. Vet Parasitol. 2011; 180:191-6.
• [3]Irwin PJ. Canine babesiosis. Vet Clin North Am Small Anim Pract. 2010; 40:1141-56.
• [4]Imre M, Farkas R, Ilie M, Imre K, Hotea I, Morariu S et al.. Seroprevalence of Babesia canis infection in clinically healthy dogs from western Romania. J Parasitol. 2013; 99:161-3.
• [5]Pennisi MG, Caprì A, Solano-Gallego L, Lombardo G, Torina A, Masucci M. Prevalence of antibodies against Rickettsia conorii, Babesia canis, Ehrlichia canis, and Anaplasma phagocytophilum antigens in dogs from the Stretto di Messina area (Italy). Tick Borne Dis. 2012; 3:315-8.
• [6]Halos L, Lebert I, Abrial D, Danlois F, Garzik K, Rodes D et al.. Questionnaire-based survey on the distribution and incidence of canine babesiosis in countries of Western Europe. Parasite. 2014; 13:1-7.
• [7]Schoeman JP. Canine babesiosis. Onderstepoort J Vet Res. 2009; 76:59-66.
• [8]Schaarschmidt D, Gilli U, Gottstein B, Marreros N, Kuhnert P, Daeppen JA et al.. Questing Dermacentor reticulatus harbouring Babesia canis DNA associated with outbreaks of canine babesiosis in the Swiss Midlands. Ticks Tick Borne Dis. 2013; 4:334-40.
• [9]Matjila TP, Nijhof AM, Taoufik A, Houwers D, Teske E, Penzhorn BL et al.. Autochthonous canine babesiosis in The Netherlands. Vet Parasitol. 2005; 131:23-9.
• [10]Bonneau S, Fourie JJ, Rousseau C, Cadiergues MC. Comparative efficacy of two fipronil spot-on formulation against experimental flea infestations (Ctenocephalides felis) in dogs. Intern J Appl Res Vet Med. 2010; 8:16-20.
• [11]Podaliri VM, Iannetti L, Paganico D, Iannino F, Ferri N. Methods of Control of the Leishmania infantum Dog Reservoir: State of the Art. Vet Med Int. 2011; 2011:Article ID 215964.
• [12]Endris RG, Matthewson MD, Cooke D, Amodie D. Repellency and efficacy of 65% permethrin and 9.7% fipronil against Ixodes ricinus. Vet Therapeut. 2000; 1:159-68.
• [13]Endris RG, Cooke D, Amodie D, Sweeney DL, Katz TL. Repellency and efficacy of 65% permethrin and selamectin spot-on formulations against lxodes ricinus ticks on dogs. Vet Therapeut. 2002; 3:64-71.
• [14]Fourie JJ, Stanneck D, Jongejan F. Prevention of transmission of Babesia canis by Dermacentor reticulatus ticks to dogs treated with an imidacloprid/flumethrin collar. Vet Parasitol. 2013; 192:273-8.
• [15]Jongejan F, Fourie JJ, Chester ST, Manavella C, Mallouk Y, Pollmeier MG et al.. The prevention of transmission of Babesia canis canis by Dermacentor reticulatus ticks to dogs using a novel combination of fipronil, amitraz and (S)-methoprene. Vet Parasitol. 2011; 179:343-50.
• [16]Marchiondo AA, Holdsworth PA, Fourie LJ, Rugg D, Hellmann K, Snyder DE et al.. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition: guidelines for evaluating the efficacy of parasiticides for the treatment, prevention and control of flea and tick infestations on dogs and cats. Vet Parasitol. 2013; 194:84-97.
• [17]Beugnet F, Halos L, Larsen D, Labuschagné M, Erasmus H, Fourie J. The ability of an oral formulation of afoxolaner to block the transmission of Babesia canis by Dermacentor reticulatus ticks to dogs. Parasit Vectors. 2014; 7:283. BioMed Central Full Text
• [18]Guideline 9: guideline on good clinical practices. CVMP/VICH/595/98-Final. 2000.
• [19]Guideline for the testing and evaluation of the efficacy of antiparasitic substances for the treatment and prevention of tick and flea infestation in dogs and cats. EMEA/CVNP/EWP/005/2000-Rev.2. 2007.
• [20]Beelitz P, Schumacher S, Marholdt F, Pfister K, Silaghi C. The prevalence of Babesia canis canis in marsh ticks (Dermacentor reticulatus) in the Saarland. Berl Munch Tierarztl Wochenschr. 2012; 125:168-71.
• [21]Maggi RG, Birkenheuer AJ, Hegarty BC, Bradley JM, Levy MG, Breitschwerdt EB. Comparison of serological and molecular panels for diagnosis of vector-borne diseases in dogs. Parasit Vectors. 2014; 7:127. BioMed Central Full Text
• [22]Bonneau S, Gupta S, Maynard L, Eun HM. Antiparasitic efficacy of a new fipronil-based spot-on formulation on dogs experimentally infested with ticks Dermacentor reticulatus. Intern J Appl Res Vet Med. 2011; 9:1-5.
• [23]Lüssenhop J, Baümer W, Kietzmann M, Schnieder T, Wolken S. Dynamics of distribution and efficacy of different spot-on permethrin formulations in dogs artificially infested with Dermacentor reticulatus. Parasit Vectors. 2011; 4:45. BioMed Central Full Text
• [24]Last RD, Hill JM, Matjila PT, Rème CA. A field trial evaluation of the prophylactic efficacy of amitraz-impregnated collars against canine babesiosis (Babesia canis rossi) in South Africa. J S Afr Vet Assoc. 2007; 78:63-5.