Physicochemical characterization of vibriophage N5

Virology Journal

Amar N Ghosh²  Anindito Sen¹ 

[1] (Present Address)Laboratory of Structural Biology, Room 1504, Building 50, NIAMS/NIH Bethesda, MD, 20852, USA;Division of Electron Microscopy, National Institute of Cholera and Enteric Diseaess, P-33, C.I.T. Road, Scheme- XM, Beleghata, Kolkata- 700010. India

关键词: Bacteriophage;    Partial denaturation mapping;    Electron Microscopy;    Vibrio cholerae;    DNA;    Vibriophage N5;   

Others  :  1158192
DOI  :  10.1186/1743-422X-2-27

 received in 2004-12-20, accepted in 2005-04-11,  发布年份 2005

【 摘 要 】

Phage N5 is one of the phages of Vibrio cholerae serovar O1 biotype El Tor (Ghosh, A. N., Ansari, M. Q., and Dutta, G. C. Isolation and morphological characterization of El Tor cholera phages. J. Gen. Virol. 70: 2241–2243, 1989). In the present communication the growth curve, molecular weight and confirmation of the genome, partial denaturation map and restriction endonuclease digestion pattern have been determined. Partial denaturation map indicates that the genome has non-permuted / invariant sequence. Presence of cohesive ends has also been documented.

【 授权许可】

   
2005 Sen and Ghosh; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Mukherjee S: Principles and practice of typing Vibrio cholerae. Methods Microbiol 1978, 12:51-115.
• [2]Guidolin A, Manning PA: Genetics of Vibrio cholerae and its bacteriophages. Microbiol Rev 1987, 51:285-298.
• [3]Ghosh AN, Ansari MQ, Dutta GC: Isolation and morphological characterization of El Tor cholera phages. J Gen Virol 1989, 70:2241-2243.
• [4]Adams MH: Bacteriophages. Interscience publishers, Inc. New York; 1959.
• [5]Chakrabarti BK, Chattopadhyay DJ, Ghosh AN: Vibriophage D10 contains non-permutated DNA with cohesive ends. J Gen Virol 1993, 74:2749-2752.
• [6]Ghosh AN, Chakrabarti BK, Chattopadhyay DJ, Sil S: Vibriophage N4 DNA is nonpermutated and terminally redundant. Can J Microbiol 1995, 41:842-845.
• [7]Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
• [8]Sambrook J, Fritsch EF, Maniatis T: Bacteriophage λ growth, purification and DNA extraction.2.60–2.81, and SDS-polyacrylamide gel electrophoresis of proteins, 18.47–18.59. In Molecular cloning: a laboratory manual. 2nd edition. Edited by Ford N, Nolan C, Ferguson M. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York; 1989.
• [9]Basu R, Ghosh AN, Ghosh A: Biophysical characterization of Vibrio El Tor typing phage e5. FEMS Microbiol Lett 1993, 106:9-16.
• [10]Kleinschmidth AK, Lang D, Jacherts D, Zhan RK: Darstellung und Längenmessungen des gesamten Desoxyriobnuclein-Säure-Inhaltes von T2 bakteriophagen. Biochim Biophys Acta 1962, 61:857-869.
• [11]Inman RB: Partial denatuartion mapping of DNA determined by electron microscopy. In Electron microscopy in biology. Volume 2. Edited by Griffith JD. John Wiley and sons, New York; 1982::237-271.
• [12]Tye BK, Huberman JA, Botstein D: Non-random circular permutation of phage P22 DNA. J Mol Biol 1974, 85:501-528.
• [13]Davis R, Simon M, Davidson N: Electron microscope for hetroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods Enzymol 1971, 21:413-428.