Hypothalamic inflammation is reversed by endurance training in anorectic-cachectic rats

Nutrition & Metabolism

Marília Seelaender⁴  Filippo Rossi-Fanelli³  Alessandro Laviano³  Miguel L Batista⁴  Ronaldo VT Santos²  Gustavo D Pimentel¹  Luiz C Carnevali⁴  Erico Caperuto⁴  Fábio L Tavares⁴  Jose C Rosa¹  Alex S Yamashita⁴  Fábio S Lira¹ 

[1] Department of Physiology, Division of Nutrition Physiology, Federal University of São Paulo (UNIFESP), São Paulo, SP - Brazil;Department of Bioscience, Federal University of São Paulo, Baixada Santista, Campus, São Paulo, Brazil;Department of Clinical Medicine, Sapienza University of Rome, Rome - Italy;Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP - Brazil

关键词: inflammation;    cytokines;    exercise training;    hypothalamus;    cancer cachexia;    anorexia;   

Others  :  820379
DOI  :  10.1186/1743-7075-8-60

 received in 2011-05-06, accepted in 2011-08-24,  发布年份 2011

【 摘 要 】

Aim

We tested the effects of a cancer cachexia-anorexia sydrome upon the balance of anti and pro-inflammatory cytokines in the hypothalamus of sedentary or trained tumour-bearing (Walker-256 carcinosarcoma) rats.

Methods

Animals were randomly assigned to a sedentary control (SC), sedentary tumour-bearing (ST), and sedentary pair-fed (SPF) groups or, exercised control (EC), exercised tumour-bearing (ET) and exercised pair-fed (EPF) groups. Trained rats ran on a treadmill (60%VO_2max) for 60 min/d, 5 days/wk, for 8 wks. We evaluated food intake, leptin and cytokine (TNF-α, IL1β) levels in the hypothalamus.

Results

The cumulative food intake and serum leptin concentration were reduced in ST compared to SC. Leptin gene expression in the retroperitoneal adipose tissue (RPAT) was increased in SPF in comparison with SC and ST, and in the mesenteric adipose tissue (MEAT) the same parameter was decreased in ST in relation to SC. Leptin levels in RPAT and MEAT were decreased in ST, when compared with SC. Exercise training was also able to reduce tumour weight when compared to ST group. In the hypothalamus, IL-1β and IL-10 gene expression was higher in ST than in SC and SPF. Cytokine concentration in hypothalamus was higher in ST (TNF-α and IL-1β, p < 0.05), compared with SC and SPF. These pro-inflammatory cytokines concentrations were restored to control values (p < 0.05), when the animals were submitted to endurance training.

Conclusion

Cancer-induced anorexia leads towards a pro-inflammatory state in the hypothalamus, which is prevented by endurance training which induces an anti-inflammatory state, with concomitant decrease of tumour weight.

【 授权许可】

   
2011 Lira et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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