Spontaneous antral follicle formation and metaphase II oocyte from a non-stimulated prepubertal ovarian tissue xenotransplant

Reproductive Biology and Endocrinology

Ralf Dittrich²  Dagmar Töpfer³  Hans van der Ven¹  Matthias W Beckmann²  Inge Hoffmann²  Markus Montag⁴  Stephanie M Nichols-Burns²  Jana Liebenthron¹  Laura Lotz² 

[1] Department of Gynecologic Endocrinology and Reproductive Medicine, University Women’s Hospital, Bonn, Germany;Department of Obstetrics and Gynecology, Erlangen University Hospital, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany;Department of Reproductive Biology, University of Veterinary Medicine Hannover, Foundation, Buenteweg 2, 30559 Hannover, Germany;ilabcomm GmbH, Eisenachstr. 34, 53757 St. Augustin, Germany

关键词: Metaphase II oocyte;    Xenotransplantation;    Prepubertal ovarian tissue;    Ovarian tissue cryopreservation;    Fertility preservation;   

Others  :  803747
DOI  :  10.1186/1477-7827-12-41

 received in 2014-02-11, accepted in 2014-04-26,  发布年份 2014

【 摘 要 】

Background

Current strategies in cancer treatment have markedly increased the rates of remission and survival for cancer patients, but are often associated with subsequent sterility. While there are various options available to an adult female depending on the patient’s particular situation, the only realistic option for preserving fertility in prepubertal females is to cryopreserve ovarian tissue. This is the first report of a morphologically mature oocyte collected from non-stimulated prepubertal ovarian tissue xenotransplants.

Methods

Ovarian tissue from a 6 year old patient suffering from nephroblastoma was removed and cryopreserved for fertility preservation. The frozen-thawed ovarian tissue fragments were xenotransplanted to bilaterally oophorectomized severe combined immunodeficiency (SCID) mice to assess follicle development.

Results

Antral follicle formation occurred post-xenotransplantation in a single ovarian fragment without exogenous hormone stimulation. A morphologically maturing oocyte was harvested from these follicles.

Conclusions

Prepubertal human ovarian follicles and oocytes can be matured after xenotransplantation even without exogenous hormone stimulation. These results indicate that tissue collected from prepubertal patients can support fertility in cancer survivors.

【 授权许可】

   
2014 Lotz et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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